Anti-RING2 / RING1B / RNF2 antibody (ab101273)
Key features and details
- Rabbit polyclonal to RING2 / RING1B / RNF2
- Suitable for: WB, IP, ICC/IF, IHC-P, ChIP-sequencing
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-RING2 / RING1B / RNF2 antibody
See all RING2 / RING1B / RNF2 primary antibodies -
Description
Rabbit polyclonal to RING2 / RING1B / RNF2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IF, IHC-P, ChIP-sequencingmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus -
Immunogen
Synthetic peptide corresponding to Human RING2/ RING1B/ RNF2 aa 150-250.
Database link: Q99496 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 37 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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ICC/IF | (1) |
Use at an assay dependent concentration.
Use with methanol or paraformaldehyde fixed cells. |
IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ChIP-sequencing |
Use at an assay dependent concentration.
10 µg |
Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 37 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ICC/IF
Use at an assay dependent concentration. Use with methanol or paraformaldehyde fixed cells. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ChIP-sequencing
Use at an assay dependent concentration. 10 µg |
Target
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Function
E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 RING-type zinc finger. -
Post-translational
modificationsPolyubiquitinated in the presence of UBE2D3 (in vitro).
Monoubiquitinated, by auto-ubiquitination. -
Cellular localization
Nucleus. Chromosome. Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells. - Information by UniProt
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Database links
- Entrez Gene: 540090 Cow
- Entrez Gene: 480042 Dog
- Entrez Gene: 6045 Human
- Entrez Gene: 19821 Mouse
- Entrez Gene: 100174504 Orangutan
- Entrez Gene: 304850 Rat
- Omim: 608985 Human
- SwissProt: Q99496 Human
see all -
Alternative names
- BAP 1 antibody
- BAP1 antibody
- DING antibody
see all
Images
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All lanes : Anti-RING2 / RING1B / RNF2 antibody (ab101273) at 0.1 µg/ml
Lane 1 : K562 whole cell lysate at 50 µg
Lane 2 : K562 whole cell lysate at 15 µg
Lane 3 : K562 whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling RING2 / RING1B / RNF2 with ab101273 at 1/1000 (1µg/ml).
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Detection of RING2 / RING1B / RNF2 in Immunoprecipitates of K562 whole cell lysate (1 mg for IP, 20% of IP loaded) using ab101273 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab101273 has been referenced in 18 publications.
- Desai D & Pethe P ChIP-qPCR for Polycomb Group Proteins During Neuronal Differentiation of Human Pluripotent Stem Cells. Methods Mol Biol 2520:117-133 (2022). PubMed: 33945141
- Sawai A et al. PRC1 sustains the integrity of neural fate in the absence of PRC2 function. Elife 11:N/A (2022). PubMed: 34994686
- Mizukoshi M et al. In vivo cell proliferation analysis and cell-tracing reveal the global cellular dynamics of periodontal ligament cells under mechanical-loading. Sci Rep 11:9813 (2021). PubMed: 33963224
- Bakhoum MF et al. Loss of polycomb repressive complex 1 activity and chromosomal instability drive uveal melanoma progression. Nat Commun 12:5402 (2021). PubMed: 34518527
- Stielow B et al. The SAM domain-containing protein 1 (SAMD1) acts as a repressive chromatin regulator at unmethylated CpG islands. Sci Adv 7:N/A (2021). PubMed: 33980486