Key features and details
- Rabbit polyclonal to RING2 / RING1B / RNF2
- Suitable for: ChIP, IP, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-RING2 / RING1B / RNF2 antibody
See all RING2 / RING1B / RNF2 primary antibodies
DescriptionRabbit polyclonal to RING2 / RING1B / RNF2
Tested applicationsSuitable for: ChIP, IP, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human RING2/ RING1B/ RNF2 aa 1-336.
MSQAVQTNGTQPLSKTWELSLYELQRTPQEAITDGLEIVVSPRSLHSELM CPICLDMLKNTMTTKECLHRFCADCIITALRSGNKECPTCRKKLVSKRSL RPDPNFDALISKIYPSRDEYEAHQERVLARINKHNNQQALSHSIEEGLKI QAMNRLQRGKKQQIENGSGAEDNGDSSHCSNASTHSNQEAGPSNKRTKTS DDSGLELDNNNAAMAIDPVMDGASEIELVFRPHPTLMEKDDSAQTRYIKT SGNATVDHLSKYLAVRLALEELRSKGESNQMNLDTASEKQYTIYIATASG QFTVLNGSFSLELVSEKYWKVNKPMELYYAPTKEHK
Database link: Q99496
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab187509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||1/50 - 1/200.|
|IP||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 37 kDa.|
|IHC-P||1/50 - 1/200.|
FunctionE3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 RING-type zinc finger.
modificationsPolyubiquitinated in the presence of UBE2D3 (in vitro).
Monoubiquitinated, by auto-ubiquitination.
Cellular localizationNucleus. Chromosome. Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells.
- Information by UniProt
- BAP 1 antibody
- BAP1 antibody
- DING antibody
Paraffin-embedded human gastric cancer tissue stained for RING2 / RING1B / RNF2 using ab187509 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-RING2 / RING1B / RNF2 antibody (ab187509) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : U-937 cell lysate
Lane 4 : SH-SY5Y cell lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : Mouse spleen tissue lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 90 seconds
Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 200μg extracts of 293T cells using 1μg of ab187509. Western blot was performed from the immunoprecipitate using ab187509 at a dilution of 1:1000.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using ab187509 and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Paraffin-embedded mouse brain tissue stained for RING2 / RING1B / RNF2 using ab187509 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded rat lung tissue stained for RING2 / RING1B / RNF2 using ab187509 at 1/100 dilution in immunohistochemical analysis.
ab187509 has not yet been referenced specifically in any publications.