Recombinant Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)
![Western blot - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)](https://www.abcam.com/ps/products/234/ab234421/Images/ab234421-1-anti-ring2-ring1b-rnf2-phospho-s168-antibody-epr20902-58-western-blot.jpg "Western blot - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20902-58] to RING2 / RING1B / RNF2 (phospho S168)
- Suitable for: WB, IP, Dot blot
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58]
See all RING2 / RING1B / RNF2 primary antibodies -
Description
Rabbit monoclonal [EPR20902-58] to RING2 / RING1B / RNF2 (phospho S168) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Dot blotmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Overexpression of wild type RNF2 in RNF2 knock out mouse embryonic stem cells derived from C57BL/6 mouse, whole cell lysate; NCCIT, HEK-293T, HepG2 and F9 whole cell lysates; mouse testis tissue lysate. Dot blot: RNF2 (phospho S168) peptide (aa161-172) and RNF2 (phospho S168) peptide (aa165-176). IP: F9 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20902-58 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
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| IP |
1/30.
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| Dot blot |
1/1000.
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa). |
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IP
1/30. |
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Dot blot
1/1000. |
Target
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Function
E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 RING-type zinc finger. -
Post-translational
modificationsPolyubiquitinated in the presence of UBE2D3 (in vitro).
Monoubiquitinated, by auto-ubiquitination. -
Cellular localization
Nucleus. Chromosome. Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells. - Information by UniProt
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Database links
- Entrez Gene: 6045 Human
- Entrez Gene: 19821 Mouse
- Omim: 608985 Human
- SwissProt: Q99496 Human
- SwissProt: Q9CQJ4 Mouse
- Unigene: 591490 Human
- Unigene: 482250 Mouse
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Alternative names
- BAP 1 antibody
- BAP1 antibody
- DING antibody
see all
Images
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Western blot - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)All lanes : Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421) at 1/1000 dilution
Lane 1 : Overexpression of wild type RNF2 in RNF2 knock out mouse embryonic stem cells derived from C57BL/6 mouse, whole cell lysate
Lane 2 : Overexpression of S168A mutant RNF2 in RNF2 knock out mouse embryonic stem cells derived from C57BL/6 mouse, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Lysates were kindly provided by Dr Jinzhong Qin.
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![Dot Blot - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)](https://www.abcam.com/ps/products/234/ab234421/Images/ab234421-5-anti-ring2-ring1b-rnf2-phospho-s168-antibody-epr20902-58-dot-mouse.jpg)
Dot Blot - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)Dot blot analysis of RING2 / RING1B / RNF2 (phospho S168) peptide labeled with ab234421 at 1/1000 dilution.
Lane 1: RING2 / RING1B / RNF2 (phospho S168) peptide (aa161-172).
Lane 2: RING2 / RING1B / RNF2 (phospho S168) peptide (aa165-176).
Lane 3: RING2 / RING1B / RNF2 non-phospho peptide (aa161-172).Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Immunoprecipitation - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)](https://www.abcam.com/ps/products/234/ab234421/Images/ab234421-4-anti-ring2-ring1b-rnf2-phospho-s168-antibody-epr20902-58-immunoprecipitation-f9-mouse.jpg)
Immunoprecipitation - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)RING2 / RING1B / RNF2 (phospho S168) was immunoprecipitated from 0.35 mg of F9 (mouse embryonic testicular cancer cell line) whole cell lysate with a234421 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab234421 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
Lane 1: F9 whole cell lysate 10 μg (Input).
Lane 2: ab234421 IP in F9 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234421 in F9 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds. -
![Western blot - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)](https://www.abcam.com/ps/products/234/ab234421/Images/ab234421-3-anti-ring2-ring1b-rnf2-phospho-s168-antibody-epr20902-58-western-blot-human-mouse.jpg)
Western blot - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)All lanes : Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : F9 (mouse embryonic testicular cancer cell line) whole cell lysate
Lane 6 : Mouse testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lane 1: 3 minutes; Lanes 2 & 3: 15 seconds; Lanes 4-6: 3 minutes.
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![Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)](https://www.abcam.com/ps/products/234/ab234421/Images/ab234421-6-benefits-of-recombinant-antibodies.png)
Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab234421 has not yet been referenced specifically in any publications.