Overview

  • Product name

  • Description

    Rabbit polyclonal to RIO2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Zebra finch
  • Immunogen

    Synthetic peptide within Human RIO2 aa 275-325. The exact sequence is proprietary. NP_060813.1

  • Positive control

    • WB: Whole cell lysate from HeLa cells. IP: Whole cell lysate from HeLa cells.
  • General notes

    Protein previously known as RIOK2.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 63 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Sequence similarities

    Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ11159 antibody
    • OTTHUMP00000158911 antibody
    • OTTHUMP00000222868 antibody
    • RIO kinase 2 (yeast) antibody
    • RIO kinase 2 antibody
    • RIO2 antibody
    • RIOK2 antibody
    • RIOK2_HUMAN antibody
    • Serine/threonine protein kinase RIO2 antibody
    • Serine/threonine-protein kinase rio2 antibody
    see all

Images

  • All lanes : Anti-RIO2 antibody (ab88485) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Predicted band size: 63 kDa

  • 3 µg ab88485 were used to immunoprecipitate 1 mg HeLa whole cell lysate. 20% of immunoprecipitate was loaded on gel and probed with ab88485 at 1 µg/ml.

References

This product has been referenced in:

  • Weinberg F  et al. The Atypical Kinase RIOK1 Promotes Tumor Growth and Invasive Behavior. EBioMedicine N/A:N/A (2017). WB ; Human . Read more (PubMed: 28499923) »
See 1 Publication for this product

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