Anti-RIOK1/RIO1 antibody (ab88496)
Key features and details
- Rabbit polyclonal to RIOK1/RIO1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RIOK1/RIO1 antibody
See all RIOK1/RIO1 primary antibodies -
Description
Rabbit polyclonal to RIOK1/RIO1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan, Bat, Elephant -
Immunogen
Synthetic peptide corresponding to Human RIOK1/RIO1.
Database link: NP_113668.2 -
Positive control
- Whole cell lysate from HeLa or 293T cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 66 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Sequence similarities
Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 83732 Human
- SwissProt: Q9BRS2 Human
- Unigene: 437474 Human
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Alternative names
- AD034 antibody
- bA288G3.1 antibody
- RIO kinase 1 (yeast) antibody
see all
Images
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All lanes : Anti-RIOK1/RIO1 antibody (ab88496) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 66 kDa
Exposure time: 3 minutes -
3ug ab88496 were used to immunoprecipitate 1mg HeLa whole cell lysate. 20% of immunoprecipitate was loaded on gel and probed with ab88496 at 0.4ug/ml.
Detection: chemoluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab88496 has been referenced in 3 publications.
- Jha SK et al. KLK3/PSA and cathepsin D activate VEGF-C and VEGF-D. Elife 8:N/A (2019). PubMed: 31099754
- Hörmann A et al. RIOK1 kinase activity is required for cell survival irrespective of MTAP status. Oncotarget 9:28625-28637 (2018). PubMed: 29983885
- Weinberg F et al. The Atypical Kinase RIOK1 Promotes Tumor Growth and Invasive Behavior. EBioMedicine N/A:N/A (2017). WB ; Human . PubMed: 28499923