Overview

  • Product name
    Anti-RIOK1 antibody - N-terminal
    See all RIOK1 primary antibodies
  • Description
    Rabbit polyclonal to RIOK1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RIOK1 aa 134-163 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9BRS2

  • Positive control
    • K562 or transfected 293 lysate, Human lung carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab176005 was purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 66 kDa.

Target

  • Sequence similarities
    Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • AD034 antibody
    • bA288G3.1 antibody
    • RIO kinase 1 (yeast) antibody
    • RIO kinase 1 antibody
    • RIOK1 antibody
    • RIOK1_HUMAN antibody
    • Serine/threonine-protein kinase rio1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labeling RIOK1 with ab176005 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • All lanes : Anti-RIOK1 antibody - N-terminal (ab176005) at 1/1000 dilution

    Lane 1 : Non-transfected 293 lysate
    Lane 2 : Transfected 293 lysate

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 66 kDa

  • Anti-RIOK1 antibody - N-terminal (ab176005) at 1/1000 dilution + K562 lysate at 35 µg

    Predicted band size: 66 kDa

References

ab176005 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab176005.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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