Product nameAnti-RIOK1/RIO1 antibody
See all RIOK1/RIO1 primary antibodies
DescriptionRabbit polyclonal to RIOK1/RIO1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan, Bat, Elephant
Synthetic peptide corresponding to Human RIOK1/RIO1.
Database link: NP_113668.2
- Whole cell lysate from HeLa or 293T cells
This product was previously labelled as RIOK1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab88496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 66 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
Sequence similaritiesBelongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.
Contains 1 protein kinase domain.
- Information by UniProt
- AD034 antibody
- bA288G3.1 antibody
- RIO kinase 1 (yeast) antibody
All lanes : Anti-RIOK1/RIO1 antibody (ab88496) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 66 kDa
Exposure time: 3 minutes
3ug ab88496 were used to immunoprecipitate 1mg HeLa whole cell lysate. 20% of immunoprecipitate was loaded on gel and probed with ab88496 at 0.4ug/ml.
Detection: chemoluminescence with exposure time of 30 seconds.
This product has been referenced in:
- Jha SK et al. KLK3/PSA and cathepsin D activate VEGF-C and VEGF-D. Elife 8:N/A (2019). Read more (PubMed: 31099754) »
- Hörmann A et al. RIOK1 kinase activity is required for cell survival irrespective of MTAP status. Oncotarget 9:28625-28637 (2018). Read more (PubMed: 29983885) »