Key features and details
- Rabbit polyclonal to RIOK3
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RIOK3 antibody
See all RIOK3 primary antibodies
DescriptionRabbit polyclonal to RIOK3
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human RIOK3 aa 469-519. The exact sequence is proprietary.
Database link: O14730
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241361 was affinity purified using an epitope specific to RIOK3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Use 20 µl/mg lysate.
|WB||1/1000. Predicted molecular weight: 59 kDa.|
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.
Contains 1 protein kinase domain.
- Information by UniProt
- Homolog of the Aspergillus nidulans sudD gene product antibody
- RIO kinase 3 (yeast) antibody
- RIO kinase 3 antibody
All lanes : Anti-RIOK3 antibody (ab241361) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 59 kDa
Exposure time: 75 seconds
RIOK3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241361 at 20 µl/reaction. Western blot was performed from the immunoprecipitate using ab241361 at 1/250 dilution.
Lane 1: ab241361 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Lysate prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241361 has not yet been referenced specifically in any publications.