Western blot abreview for Anti-RIP antibody [7H10]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Embryonic Kidney Fibroblasts)
Gel Running Conditions
Reduced Non-Denaturing (Native) (10%)
Loading amount
1e+006 cells
Specification
Embryonic Kidney Fibroblasts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: TBST
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: LiCOR anti Mouse IRdye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Odyssey scanner
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 75 kDa

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Submitted Mar 25 2016

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