Anti-RIP antibody (ab106393)
Key features and details
- Rabbit polyclonal to RIP
- Suitable for: WB, ICC, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-RIP antibody
See all RIP primary antibodies -
Description
Rabbit polyclonal to RIP -
Host species
Rabbit -
Specificity
ab106393 reacts with RIP. It does not crossreact with other TKL Ser/Thr protein kinase family members. -
Tested applications
Suitable for: WB, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human RIP (N terminal).
Database link: Q13546 -
Positive control
- WB: Rat kidney lysate IHC-P: Mouse kidney tissue ICC: Mouse kidney cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 76 kDa.
Validated in rat only |
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| ICC |
Use a concentration of 20 µg/ml.
Validated in mouse only |
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| IHC-P |
Use a concentration of 2.5 µg/ml.
Validated in mouse only |
| Notes |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 76 kDa. Validated in rat only |
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ICC
Use a concentration of 20 µg/ml. Validated in mouse only |
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IHC-P
Use a concentration of 2.5 µg/ml. Validated in mouse only |
Target
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Function
Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 death domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsProteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction.
Autophosphorylated on serine and threonine residues.
Ubiquitinated by 'Lys-11'-, 'Lys-48'-, 'Lys-63'- and linear-linked type ubiquitin. Polyubiquitination with 'Lys-63'-linked chains by TRAF2 induces association with the IKK complex. Deubiquitination of 'Lys-63'-linked chains and polyubiquitination with 'Lys-48'-linked chains by TNFAIP3 leads to RIPK1 proteasomal degradation and consequently to the termination of the TNF- or Linear polyubiquitinated; the head-to-tail polyubiquitination is mediated by the LUBAC complex. LPS-mediated activation of NF-kappa-B. Also ubiquitinated with 'Lys-11'-linked chains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 19766 Mouse
- Entrez Gene: 306886 Rat
- SwissProt: Q60855 Mouse
- Unigene: 374799 Mouse
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Alternative names
- Cell death protein RIP antibody
- FLJ39204 antibody
- OTTHUMP00000039163 antibody
see all
Images
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Western blot - Anti-RIP antibody (ab106393)Anti-RIP antibody (ab106393) at 1 µg/ml + Rat kidney tissue lysate at 15 µg
Predicted band size: 76 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIP antibody (ab106393)Immunohistochemical analysis of mouse kidney tissue section labelling RIP with ab106393 at 2.5 ug/mL.
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Immunocytochemistry/ Immunofluorescence - Anti-RIP antibody (ab106393)Immunofluorescence of RIP in Mouse Kidney cells using ab106393 at 20 ug/ml.
Protocols
Datasheets and documents
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Datasheet download
References (13)
ab106393 has been referenced in 13 publications.
- Yin Z et al. RIPK1 is a negative mediator in Aquaporin 1-driven triple-negative breast carcinoma progression and metastasis. NPJ Breast Cancer 7:53 (2021). PubMed: 33980862
- Lin QS et al. RIP1/RIP3/MLKL mediates dopaminergic neuron necroptosis in a mouse model of Parkinson disease. Lab Invest 100:503-511 (2020). PubMed: 31506635
- Su W et al. Dose- and Time-Dependent Cytotoxicity of Carteolol in Corneal Endothelial Cells and the Underlying Mechanisms. Front Pharmacol 11:202 (2020). PubMed: 32210806
- Shen F et al. Pharmacological Inhibition of Necroptosis Promotes Human Breast Cancer Cell Proliferation and Metastasis. Onco Targets Ther 13:3165-3176 (2020). PubMed: 32368076
- Aravind A et al. Blast exposure predisposes the brain to increased neurological deficits in a model of blast plus blunt traumatic brain injury. Exp Neurol 332:113378 (2020). PubMed: 32553593
