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  1. Link

    rip-antibody-ab106393.pdf

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Cell Biology Apoptosis Intracellular Kinases
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Anti-RIP antibody (ab106393)

  • Datasheet
Reviews (3)Q&A (2)References (13)

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Western blot - Anti-RIP antibody (ab106393)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIP antibody (ab106393)
  • Immunocytochemistry/ Immunofluorescence - Anti-RIP antibody (ab106393)

Key features and details

  • Rabbit polyclonal to RIP
  • Suitable for: WB, ICC, IHC-P
  • Reacts with: Mouse, Rat
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-RIP antibody [EPR19697] (ab202985)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-RIP antibody
    See all RIP primary antibodies
  • Description

    Rabbit polyclonal to RIP
  • Host species

    Rabbit
  • Specificity

    ab106393 reacts with RIP. It does not crossreact with other TKL Ser/Thr protein kinase family members.
  • Tested applications

    Suitable for: WB, ICC, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human RIP (N terminal).
    Database link: Q13546

  • Positive control

    • WB: Rat kidney lysate IHC-P: Mouse kidney tissue ICC: Mouse kidney cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • RIP
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • RIP
    • Cancer
    • Cell Death
    • Necroptosis
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human RIP protein (Active) (ab190411)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab106393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 76 kDa.

Validated in rat only

ICC
Use a concentration of 20 µg/ml.

Validated in mouse only

IHC-P
Use a concentration of 2.5 µg/ml.

Validated in mouse only

Notes
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 76 kDa.

Validated in rat only

ICC
Use a concentration of 20 µg/ml.

Validated in mouse only

IHC-P
Use a concentration of 2.5 µg/ml.

Validated in mouse only

Target

  • Function

    Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction.
    Autophosphorylated on serine and threonine residues.
    Ubiquitinated by 'Lys-11'-, 'Lys-48'-, 'Lys-63'- and linear-linked type ubiquitin. Polyubiquitination with 'Lys-63'-linked chains by TRAF2 induces association with the IKK complex. Deubiquitination of 'Lys-63'-linked chains and polyubiquitination with 'Lys-48'-linked chains by TNFAIP3 leads to RIPK1 proteasomal degradation and consequently to the termination of the TNF- or Linear polyubiquitinated; the head-to-tail polyubiquitination is mediated by the LUBAC complex. LPS-mediated activation of NF-kappa-B. Also ubiquitinated with 'Lys-11'-linked chains.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q13546 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 19766 Mouse
    • Entrez Gene: 306886 Rat
    • SwissProt: Q60855 Mouse
    • Unigene: 374799 Mouse
    • Alternative names

      • Cell death protein RIP antibody
      • FLJ39204 antibody
      • OTTHUMP00000039163 antibody
      • Receptor (TNFRSF) interacting serine threonine kinase 1 antibody
      • receptor interacting protein 1 antibody
      • Receptor interacting protein antibody
      • Receptor interacting protein kinase 1 antibody
      • Receptor interacting serine threonine protein kinase 1 antibody
      • Receptor TNFRSF interacting serine threonine kinase 1 antibody
      • Receptor-interacting protein 1 antibody
      • Receptor-interacting serine/threonine-protein kinase 1 antibody
      • Rinp antibody
      • RIP 1 antibody
      • RIP antibody
      • Rip-1 antibody
      • RIP1 antibody
      • RIPK 1 antibody
      • Ripk1 antibody
      • RIPK1_HUMAN antibody
      • Serine threonine protein kinase RIP antibody
      • Serine/threonine-protein kinase RIP antibody
      see all

    Images

    • Western blot - Anti-RIP antibody (ab106393)
      Western blot - Anti-RIP antibody (ab106393)
      Anti-RIP antibody (ab106393) at 1 µg/ml + Rat kidney tissue lysate at 15 µg

      Predicted band size: 76 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIP antibody (ab106393)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIP antibody (ab106393)

      Immunohistochemical analysis of mouse kidney tissue section labelling RIP with ab106393 at 2.5 ug/mL.

    • Immunocytochemistry/ Immunofluorescence - Anti-RIP antibody (ab106393)
      Immunocytochemistry/ Immunofluorescence - Anti-RIP antibody (ab106393)

      Immunofluorescence of RIP in Mouse Kidney cells using ab106393 at 20 ug/ml.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (13)

    Publishing research using ab106393? Please let us know so that we can cite the reference in this datasheet.

    ab106393 has been referenced in 13 publications.

    • Yin Z  et al. RIPK1 is a negative mediator in Aquaporin 1-driven triple-negative breast carcinoma progression and metastasis. NPJ Breast Cancer 7:53 (2021). PubMed: 33980862
    • Lin QS  et al. RIP1/RIP3/MLKL mediates dopaminergic neuron necroptosis in a mouse model of Parkinson disease. Lab Invest 100:503-511 (2020). PubMed: 31506635
    • Su W  et al. Dose- and Time-Dependent Cytotoxicity of Carteolol in Corneal Endothelial Cells and the Underlying Mechanisms. Front Pharmacol 11:202 (2020). PubMed: 32210806
    • Shen F  et al. Pharmacological Inhibition of Necroptosis Promotes Human Breast Cancer Cell Proliferation and Metastasis. Onco Targets Ther 13:3165-3176 (2020). PubMed: 32368076
    • Aravind A  et al. Blast exposure predisposes the brain to increased neurological deficits in a model of blast plus blunt traumatic brain injury. Exp Neurol 332:113378 (2020). PubMed: 32553593
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Imaging Mass Cytometry™ abreview for Anti-RIP antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Mass Cytometry
    Sample
    Human Tissue sections (Liver)
    Specification
    Liver
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 21 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RIP antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (p7 mouse brain)
    Specification
    p7 mouse brain
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Read More

    MR. Carl Hobbs

    Verified customer

    Submitted May 14 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RIP antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Kidney and brain)
    Specification
    Kidney and brain
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acd
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Read More

    MR. Carl Hobbs

    Verified customer

    Submitted May 14 2012

    Question

    Please send a range of amino acids that contains the immunogen sequence, to help us assess the utility of the antibody for our experiments.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 18 2013

    Answer

    The immunogen is between amino acids 181-240 of accession number NP_003795.

    Read More

    Tom Ruyle

    Abcam Scientific Support

    Answered on Sep 18 2013

    Question

    Has this antibody been used in any publications?
    Seeing staining in random neurons and some blood vessels, not specific to hippocampus as expected.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 24 2012

    Answer

    Thank you for your call on Friday and for letting us know about the trouble with this antibody.

    I've checked with the lab, and unfortunately we are not aware of any publications in which this antibody was used. Would you be able to forward me the article that reported RIP staining only in the ipsilateral hippocampus? Could you send any images of your results?

    We currently have only tested the antibody on formalin-fixed, paraffin-embedded tissue, so we don't have anoptimized staining protocol for cryosections. However I may have some further suggestions after reviewing the images and the literature.

    I look forward to hearing from you. Please let me know if you have further questions or comments, or if there is anything else that we can do for you.

    Read More

    Abcam Scientific Support

    Answered on Jul 24 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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