Anti-RIP antibody (ab106393)
Overview
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Product name
Anti-RIP antibody
See all RIP primary antibodies -
Description
Rabbit polyclonal to RIP -
Host species
Rabbit -
Specificity
ab106393 reacts with RIP. It does not crossreact with other TKL Ser/Thr protein kinase family members. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human RIP (N terminal).
Database link: Q13546 -
Positive control
- WB: Rat kidney lysate IHC-P: Mouse kidney tissue ICC/IF: Mouse kidney cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab106393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 76 kDa. | |
| IHC-P | Use a concentration of 2.5 µg/ml. |
Target
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Function
Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 death domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsProteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction.
Autophosphorylated on serine and threonine residues.
Ubiquitinated by 'Lys-11'-, 'Lys-48'-, 'Lys-63'- and linear-linked type ubiquitin. Polyubiquitination with 'Lys-63'-linked chains by TRAF2 induces association with the IKK complex. Deubiquitination of 'Lys-63'-linked chains and polyubiquitination with 'Lys-48'-linked chains by TNFAIP3 leads to RIPK1 proteasomal degradation and consequently to the termination of the TNF- or Linear polyubiquitinated; the head-to-tail polyubiquitination is mediated by the LUBAC complex. LPS-mediated activation of NF-kappa-B. Also ubiquitinated with 'Lys-11'-linked chains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 8737 Human
- Entrez Gene: 19766 Mouse
- Entrez Gene: 306886 Rat
- Omim: 603453 Human
- SwissProt: Q13546 Human
- SwissProt: Q60855 Mouse
- Unigene: 519842 Human
- Unigene: 374799 Mouse
see all -
Alternative names
- Cell death protein RIP antibody
- FLJ39204 antibody
- OTTHUMP00000039163 antibody
see all
Images
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Western blot - Anti-RIP antibody (ab106393)Anti-RIP antibody (ab106393) at 1 µg/ml + Rat kidney tissue lysate at 15 µg
Predicted band size: 76 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIP antibody (ab106393)Immunohistochemical analysis of mouse kidney tissue section labelling RIP with ab106393 at 2.5 ug/mL.
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Immunocytochemistry/ Immunofluorescence - Anti-RIP antibody (ab106393)Immunofluorescence of RIP in Mouse Kidney cells using ab106393 at 20 ug/ml.
Protocols
Datasheets and documents
References
ab106393 has been referenced in 5 publications.
- Jia J et al. Recombinant PEP-1-SOD1 improves functional recovery after neural stem cell transplantation in rats with traumatic brain injury. Exp Ther Med 15:2929-2935 (2018). PubMed: 29599832
- Wójcik M et al. Expression of receptor interacting protein 1 and receptor interacting protein 3 oval cells in a rat model of hepatocarcinogenesis. Exp Ther Med 15:4448-4456 (2018). PubMed: 29731829
- Tan H et al. MicroRNA-24-3p Attenuates Myocardial Ischemia/Reperfusion Injury by Suppressing RIPK1 Expression in Mice. Cell Physiol Biochem 51:46-62 (2018). PubMed: 30439713
- Jackson R et al. Paracrine Engineering of Human Cardiac Stem Cells With Insulin-Like Growth Factor 1 Enhances Myocardial Repair. J Am Heart Assoc 4:e002104 (2015). WB . PubMed: 26363004
- Kim JE et al. LIM kinase-2 induces programmed necrotic neuronal death via dysfunction of DRP1-mediated mitochondrial fission. Cell Death Differ : (2014). PubMed: 24561342
