Recombinant Anti-RIP antibody [EPR4689-100] (ab178420)
![Western blot - Anti-RIP antibody [EPR4689-100] (ab178420)](https://www.abcam.com/ps/products/178/ab178420/Images/ab178420-259101-anti-rip-antibody-epr4689-100-western-blot.jpg "Western blot - Anti-RIP antibody [EPR4689-100] (ab178420)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4689-100] to RIP
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RIP antibody [EPR4689-100]
See all RIP primary antibodies -
Description
Rabbit monoclonal [EPR4689-100] to RIP -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human RIP. The exact sequence is proprietary.
Database link: Q13546 -
Positive control
- HeLa cells and cell lysates; Raji cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4689-100 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 75 kDa.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 75 kDa. |
Target
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Function
Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 death domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsProteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction.
Autophosphorylated on serine and threonine residues.
Ubiquitinated by 'Lys-11'-, 'Lys-48'-, 'Lys-63'- and linear-linked type ubiquitin. Polyubiquitination with 'Lys-63'-linked chains by TRAF2 induces association with the IKK complex. Deubiquitination of 'Lys-63'-linked chains and polyubiquitination with 'Lys-48'-linked chains by TNFAIP3 leads to RIPK1 proteasomal degradation and consequently to the termination of the TNF- or Linear polyubiquitinated; the head-to-tail polyubiquitination is mediated by the LUBAC complex. LPS-mediated activation of NF-kappa-B. Also ubiquitinated with 'Lys-11'-linked chains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 8737 Human
- Omim: 603453 Human
- SwissProt: Q13546 Human
- Unigene: 519842 Human
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Alternative names
- Cell death protein RIP antibody
- FLJ39204 antibody
- OTTHUMP00000039163 antibody
see all
Images
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Western blot - Anti-RIP antibody [EPR4689-100] (ab178420)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: RIP knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Raji cell lysate (20 µg)
Lanes 1 to 4: Merged signal (red and green). Green - ab178420 observed at 78 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab178420 was shown to react with RIP in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when RIP knockout samples were examined. Wild-type and RIP knockout samples were subjected to SDS-PAGE. ab178420 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-RIP antibody [EPR4689-100] (ab178420)](https://www.abcam.com/ps/products/178/ab178420/Images/ab178420-206330-anti-rip-antibody-epr4689-100-western-blot.jpg)
Western blot - Anti-RIP antibody [EPR4689-100] (ab178420)All lanes : Anti-RIP antibody [EPR4689-100] (ab178420) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HeLa cell lysate with Staurosporine
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 75 kDa -
![Flow Cytometry (Intracellular) - Anti-RIP antibody [EPR4689-100] (ab178420)](https://www.abcam.com/ps/products/178/ab178420/Images/ab178420-206328-anti-rip-antibody-epr4689-100-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-RIP antibody [EPR4689-100] (ab178420)Intracellular flow cytometric analysis of permeabilized HeLa cells labeling RIP with ab178420 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).
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![Anti-RIP antibody [EPR4689-100] (ab178420)](https://www.abcam.com/ps/products/178/ab178420/Images/ab178420-4-benefits-of-recombinant-antibodies.png)
Anti-RIP antibody [EPR4689-100] (ab178420)
Datasheets and documents
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Datasheet download
References (1)
ab178420 has been referenced in 1 publication.
- Moriwaki K et al. The death-inducing activity of RIPK1 is regulated by the pH environment. Sci Signal 13:N/A (2020). PubMed: 32398349
