Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-RIP antibody [EPR4689-100] (ab178420)

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Western blot - Anti-RIP antibody [EPR4689-100] (ab178420)
  • Western blot - Anti-RIP antibody [EPR4689-100] (ab178420)
  • Flow Cytometry (Intracellular) - Anti-RIP antibody [EPR4689-100] (ab178420)
  • Anti-RIP antibody [EPR4689-100] (ab178420)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4689-100] to RIP
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-RIP antibody [EPR4689-100]
    See all RIP primary antibodies

  • Description

    Rabbit monoclonal [EPR4689-100] to RIP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human RIP. The exact sequence is proprietary.
    Database link: Q13546

  • Positive control

    • HeLa cells and cell lysates; Raji cell lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab178420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 75 kDa.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 75 kDa.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function

      Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains.

    • Sequence similarities

      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 1 death domain.
      Contains 1 protein kinase domain.

    • Post-translational
      modifications

      Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction.
      Autophosphorylated on serine and threonine residues.
      Ubiquitinated by 'Lys-11'-, 'Lys-48'-, 'Lys-63'- and linear-linked type ubiquitin. Polyubiquitination with 'Lys-63'-linked chains by TRAF2 induces association with the IKK complex. Deubiquitination of 'Lys-63'-linked chains and polyubiquitination with 'Lys-48'-linked chains by TNFAIP3 leads to RIPK1 proteasomal degradation and consequently to the termination of the TNF- or Linear polyubiquitinated; the head-to-tail polyubiquitination is mediated by the LUBAC complex. LPS-mediated activation of NF-kappa-B. Also ubiquitinated with 'Lys-11'-linked chains.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • Cell death protein RIP antibody
        • FLJ39204 antibody
        • OTTHUMP00000039163 antibody
        • Receptor (TNFRSF) interacting serine threonine kinase 1 antibody
        • receptor interacting protein 1 antibody
        • Receptor interacting protein antibody
        • Receptor interacting protein kinase 1 antibody
        • Receptor interacting serine threonine protein kinase 1 antibody
        • Receptor TNFRSF interacting serine threonine kinase 1 antibody
        • Receptor-interacting protein 1 antibody
        • Receptor-interacting serine/threonine-protein kinase 1 antibody
        • Rinp antibody
        • RIP 1 antibody
        • RIP antibody
        • Rip-1 antibody
        • RIP1 antibody
        • RIPK 1 antibody
        • Ripk1 antibody
        • RIPK1_HUMAN antibody
        • Serine threonine protein kinase RIP antibody
        • Serine/threonine-protein kinase RIP antibody
        see all

      Images

      • Western blot - Anti-RIP antibody [EPR4689-100] (ab178420)
        Western blot - Anti-RIP antibody [EPR4689-100] (ab178420)

        Lane 1: Wild-type HAP1 cell lysate (20 µg)
        Lane 2: RIP knockout HAP1 cell lysate (20 µg)
        Lane 3: HeLa cell lysate (20 µg)
        Lane 4: Raji cell lysate (20 µg)
        Lanes 1 to 4: Merged signal (red and green). Green - ab178420 observed at 78 kDa. Red - loading control, ab8245, observed at 37 kDa.


        ab178420 was shown to react with RIP in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when RIP knockout samples were examined. Wild-type and RIP knockout samples were subjected to SDS-PAGE. ab178420 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

      • Western blot - Anti-RIP antibody [EPR4689-100] (ab178420)
        Western blot - Anti-RIP antibody [EPR4689-100] (ab178420)
        All lanes : Anti-RIP antibody [EPR4689-100] (ab178420) at 1/1000 dilution

        Lane 1 : Raji cell lysate
        Lane 2 : HeLa cell lysate with Staurosporine
        Lane 3 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 75 kDa

      • Flow Cytometry (Intracellular) - Anti-RIP antibody [EPR4689-100] (ab178420)
        Flow Cytometry (Intracellular) - Anti-RIP antibody [EPR4689-100] (ab178420)

        Flow Cytometric analysis of permeabilized HeLa cells labeling RIP with ab178420 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).

      • Anti-RIP antibody [EPR4689-100] (ab178420)
        Anti-RIP antibody [EPR4689-100] (ab178420)

      References (1)

      Publishing research using ab178420? Please let us know so that we can cite the reference in this datasheet.

      ab178420 has been referenced in 1 publication.

      • Moriwaki K  et al. The death-inducing activity of RIPK1 is regulated by the pH environment. Sci Signal 13:N/A (2020). PubMed: 32398349

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