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Cell Biology Apoptosis Intracellular Kinases
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
  • Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
  • Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
  • Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
  • Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4689] to RIP - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

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Conjugation
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HRP Conjugation Kit - Lightning-Link® (ab102890)
Protein
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Recombinant human RIP protein (Active) (ab190411)

View more associated products

Overview

  • Product name

    Anti-RIP antibody [EPR4689] - BSA and Azide free
    See all RIP primary antibodies
  • Description

    Rabbit monoclonal [EPR4689] to RIP - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment aa 300-450 (internal sequence).

  • General notes

    ab234924 is the carrier-free version of ab125072 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab234924 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4689
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • RIP
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • RIP
    • Cancer
    • Cell Death
    • Necroptosis

Associated products

  • Alternative Versions

    • Anti-RIP antibody [EPR4689] (ab125072)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
  • Recombinant Protein

    • Recombinant human RIP protein (Active) (ab190411)

Applications

Our Abpromise guarantee covers the use of ab234924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 75 kDa.

Target

  • Function

    Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction.
    Autophosphorylated on serine and threonine residues.
    Ubiquitinated by 'Lys-11'-, 'Lys-48'-, 'Lys-63'- and linear-linked type ubiquitin. Polyubiquitination with 'Lys-63'-linked chains by TRAF2 induces association with the IKK complex. Deubiquitination of 'Lys-63'-linked chains and polyubiquitination with 'Lys-48'-linked chains by TNFAIP3 leads to RIPK1 proteasomal degradation and consequently to the termination of the TNF- or Linear polyubiquitinated; the head-to-tail polyubiquitination is mediated by the LUBAC complex. LPS-mediated activation of NF-kappa-B. Also ubiquitinated with 'Lys-11'-linked chains.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q13546 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8737 Human
    • Omim: 603453 Human
    • SwissProt: Q13546 Human
    • Unigene: 519842 Human
    • Alternative names

      • Cell death protein RIP antibody
      • FLJ39204 antibody
      • OTTHUMP00000039163 antibody
      • Receptor (TNFRSF) interacting serine threonine kinase 1 antibody
      • receptor interacting protein 1 antibody
      • Receptor interacting protein antibody
      • Receptor interacting protein kinase 1 antibody
      • Receptor interacting serine threonine protein kinase 1 antibody
      • Receptor TNFRSF interacting serine threonine kinase 1 antibody
      • Receptor-interacting protein 1 antibody
      • Receptor-interacting serine/threonine-protein kinase 1 antibody
      • Rinp antibody
      • RIP 1 antibody
      • RIP antibody
      • Rip-1 antibody
      • RIP1 antibody
      • RIPK 1 antibody
      • Ripk1 antibody
      • RIPK1_HUMAN antibody
      • Serine threonine protein kinase RIP antibody
      • Serine/threonine-protein kinase RIP antibody
      see all

    Images

    • Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
      Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)

      This data was developed using ab125072, the same antibody clone in a different buffer formulation.

      Lane 1: Wild-type HAP1 cell lysate (20 µg)

      Lane 2: RIP knockout HAP1 cell lysate (20 µg)

      Lane 3: HeLa cell lysate (20 µg)

      Lane 4: Raji cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab125072 (unpurified) observed at 78 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab125072 was shown to specifically react with RIP in wild-type HAP1 cells. No band was observed when RIP knockout samples were examined. Wild-type and RIP knockout samples were subjected to SDS-PAGE. ab125072 at a dilution of 1/1000 and ab8245 (loading control to GAPDH) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
      Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
      Anti-RIP antibody [EPR4689] (ab125072) at 1/5000 dilution (Purified) + Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 75 kDa
      Observed band size: 75 kDa



      This data was developed using ab125072, the same antibody clone in a different buffer formulation.

    • Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
      Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
      Anti-RIP antibody [EPR4689] (ab125072) at 1/1000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 75 kDa
      Observed band size: 75 kDa



      This data was developed using ab125072, the same antibody clone in a different buffer formulation.

    • Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
      Western blot - Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
      All lanes : Anti-RIP antibody [EPR4689] (ab125072) at 1/1000 dilution (unpurified)

      Lane 1 : Raji cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 75 kDa



      This data was developed using ab125072, the same antibody clone in a different buffer formulation.

       

    • Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)
      Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab234924? Please let us know so that we can cite the reference in this datasheet.

    ab234924 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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