Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-RIP antibody [EPR4689] - BSA and Azide free (ab234924)

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Overview

  • Product name

    Anti-RIP antibody [EPR4689] - BSA and Azide free
    See all RIP primary antibodies

  • Description

    Rabbit monoclonal [EPR4689] to RIP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment aa 300-450 (internal sequence).

  • General notes

    ab234924 is the carrier-free version of ab125072 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab234924 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab234924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 75 kDa.

Target

  • Function

    Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains.

  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 death domain.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction.
    Autophosphorylated on serine and threonine residues.
    Ubiquitinated by 'Lys-11'-, 'Lys-48'-, 'Lys-63'- and linear-linked type ubiquitin. Polyubiquitination with 'Lys-63'-linked chains by TRAF2 induces association with the IKK complex. Deubiquitination of 'Lys-63'-linked chains and polyubiquitination with 'Lys-48'-linked chains by TNFAIP3 leads to RIPK1 proteasomal degradation and consequently to the termination of the TNF- or Linear polyubiquitinated; the head-to-tail polyubiquitination is mediated by the LUBAC complex. LPS-mediated activation of NF-kappa-B. Also ubiquitinated with 'Lys-11'-linked chains.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cell death protein RIP antibody
      • FLJ39204 antibody
      • OTTHUMP00000039163 antibody
      • Receptor (TNFRSF) interacting serine threonine kinase 1 antibody
      • receptor interacting protein 1 antibody
      • Receptor interacting protein antibody
      • Receptor interacting protein kinase 1 antibody
      • Receptor interacting serine threonine protein kinase 1 antibody
      • Receptor TNFRSF interacting serine threonine kinase 1 antibody
      • Receptor-interacting protein 1 antibody
      • Receptor-interacting serine/threonine-protein kinase 1 antibody
      • Rinp antibody
      • RIP 1 antibody
      • RIP antibody
      • Rip-1 antibody
      • RIP1 antibody
      • RIPK 1 antibody
      • Ripk1 antibody
      • RIPK1_HUMAN antibody
      • Serine threonine protein kinase RIP antibody
      • Serine/threonine-protein kinase RIP antibody
      see all

    References

    ab234924 has not yet been referenced specifically in any publications.

    Publishing research using ab234924? Please let us know so that we can cite the reference in this datasheet.

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