Overview

  • Product name
    Anti-RIP140 antibody [2656C6a]
    See all RIP140 primary antibodies
  • Description
    Mouse monoclonal [2656C6a] to RIP140
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab53740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 129 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • Function
    Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors.
  • Domain
    Contains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors. The C-terminal LTKTNPILYYMLQK motif is required for ligand-dependent interaction with RAAR and RXRB homodimers and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB (By similarity). Contains at least four autonomous repression domains (RD1-4). RD1 functions via a histone deacetylase (HDAC)-independent mechanism, whereas RD2, RD3 and RD4 can function by HDAC-dependent or independent mechanisms, depending on cell type. RD2 is dependent on CTBP binding.
  • Post-translational
    modifications
    Acetylation regulates its nuclear translocation and corepressive activity (By similarity). Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH.
  • Cellular localization
    Nucleus. Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1.
  • Information by UniProt
  • Database links
  • Alternative names
    • NRIP 1 antibody
    • NRIP1 antibody
    • NRIP1_HUMAN antibody
    • Nuclear factor RIP 140 antibody
    • Nuclear factor RIP140 antibody
    • Nuclear receptor interacting protein 1 antibody
    • Nuclear receptor-interacting protein 1 antibody
    • Receptor interacting protein 140 antibody
    • Receptor-interacting protein 140 antibody
    • RIP 140 antibody
    • RIP140 antibody
    see all

Images

  • Anti-RIP140 antibody [2656C6a] (ab53740) + Immunizing recombinant protein

    Predicted band size: 129 kDa
    Observed band size: 41 kDa
    why is the actual band size different from the predicted?



    The band size does not correspond to the predicted protein size, as the recombinant fragment immunogen was used for the test lane

References

ab53740 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

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