Key features and details
- Mouse monoclonal [2656C6a] to RIP140
- Suitable for: WB, Dot blot
- Reacts with: Human
- Isotype: IgG1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.
Our Abpromise guarantee covers the use of ab53740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 129 kDa.|
|Dot blot||Use at an assay dependent dilution.|
FunctionModulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors.
DomainContains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors. The C-terminal LTKTNPILYYMLQK motif is required for ligand-dependent interaction with RAAR and RXRB homodimers and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB (By similarity). Contains at least four autonomous repression domains (RD1-4). RD1 functions via a histone deacetylase (HDAC)-independent mechanism, whereas RD2, RD3 and RD4 can function by HDAC-dependent or independent mechanisms, depending on cell type. RD2 is dependent on CTBP binding.
modificationsAcetylation regulates its nuclear translocation and corepressive activity (By similarity). Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH.
Cellular localizationNucleus. Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1.
- Information by UniProt
- NRIP 1 antibody
- NRIP1 antibody
- NRIP1_HUMAN antibody
Anti-RIP140 antibody [2656C6a] (ab53740) + Immunizing recombinant protein
Predicted band size: 129 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
The band size does not correspond to the predicted protein size, as the recombinant fragment immunogen was used for the test lane
ab53740 has not yet been referenced specifically in any publications.