• Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: 3% Sodium deoxycholate, 3% Triton-X-100, 0.3% Tris HCl, 15% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Receptor Interacting Protein 140 (RIP 140) has been shown to enhance estrogen receptor transcriptional activity through the AF2 activation domain in an agonist dependent manner and has also been implicated to be involved in other steroid receptor pathways, including the retinoid pathway. RIP 140 has recently been shown to interact with peroxisome proliferator-activated receptor-alpha (PPAR alpha) and has been proposed to competitively inhibit PPAR alpha interaction with Steroid Receptor Coactivator-1 (SRC-1).
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab3425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 129 kDa).Can be blocked with RIP140 peptide (ab4968).


  • Function
    Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors.
  • Domain
    Contains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors. The C-terminal LTKTNPILYYMLQK motif is required for ligand-dependent interaction with RAAR and RXRB homodimers and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB (By similarity). Contains at least four autonomous repression domains (RD1-4). RD1 functions via a histone deacetylase (HDAC)-independent mechanism, whereas RD2, RD3 and RD4 can function by HDAC-dependent or independent mechanisms, depending on cell type. RD2 is dependent on CTBP binding.
  • Post-translational
    Acetylation regulates its nuclear translocation and corepressive activity (By similarity). Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH.
  • Cellular localization
    Nucleus. Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1.
  • Information by UniProt
  • Database links
  • Alternative names
    • NRIP 1 antibody
    • NRIP1 antibody
    • NRIP1_HUMAN antibody
    • Nuclear factor RIP 140 antibody
    • Nuclear factor RIP140 antibody
    • Nuclear receptor interacting protein 1 antibody
    • Nuclear receptor-interacting protein 1 antibody
    • Receptor interacting protein 140 antibody
    • Receptor-interacting protein 140 antibody
    • RIP 140 antibody
    • RIP140 antibody
    see all


  • Western blot of recombinant Human RIP140 using ab3425.



This product has been referenced in:
  • Al-Rewashdy H  et al. Utrophin A is essential in mediating the functional adaptations of mdx mouse muscle following chronic AMPK activation. Hum Mol Genet 24:1243-55 (2015). Read more (PubMed: 25324540) »
  • Gidlund EK  et al. Rapidly elevated levels of PGC-1a-b protein in human skeletal muscle after exercise: exploring regulatory factors in a randomized controlled trial. J Appl Physiol (1985) 119:374-84 (2015). Read more (PubMed: 26089547) »
See all 11 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


Yes, the immunogen was derived from that sequence. It is in figure 1.a. of the 1995 reference:


Apparently the sequence data was modified over time to yield the current sequence at the UniProt page. Here is a link to the list of the modifications.


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The percentage identity depends on which sequence you align with the immunogen. Aligning with the sequence at the UniProt entry, http://www.uniprot.org/uniprot/P48552 returns 78%. BLAST the immunogen and the top hit is CAA59108.1, which aligns 100%.


The UniProt entry has sequence annotations that list the conflicts:

Sequence conflict: 721 – 726 NKGKSE TKGRVK in CAA59108. Ref.1

Reference 1 is
Nuclear factor RIP140 modulates transcriptional activation by the estrogen receptor.
EMBO J. 1995 Aug 1;14(15):3741-51. PMID:7641693


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Gracias por tu respuesta.

El anticuerpo ab12193 está en stock, y si el pedido se tramita en el dia de hoy, os llegaría mañana mismo.

Antes de hacerlo, me gustaría informarte de que actualmente tenemos una promoción por la que al enviar cualquier anticuerpo primario os regalamos otro primario Rabbit Monoclonal. Simplemente indica el código RABMAB-XBSMG en el próximo pedido. Para más información picha https://www.abcam.com/index.html?pageconfig=resource&rid=15447.

Si te interesa esta promoción, puedo mandarte hoy mismo en el mismo pedido el anticuerpo ab12193 de reemplazo, y otro RabMab totalmente gratuito. Indícame la referencia del RabMab que os interesaría, y procedo con el envío.

Espero tu respuesta.

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Thank you for your inquiry and I sincerely apologize for the long delay. We had to wait on the supplying lab's Marketing Manager to look back through the historic information for this antibody. The sequence that is listed on our datasheet is correct, as we did incorporate some substitutions in the sequence of the immunogen. Thank you for your patience and again I apologize that it has taken so long to resolve this inquiry.

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