Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: 3% Sodium deoxycholate, 3% Triton-X-100, 0.3% Tris HCl, 15% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Primary antibody notesSteroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Receptor Interacting Protein 140 (RIP 140) has been shown to enhance estrogen receptor transcriptional activity through the AF2 activation domain in an agonist dependent manner and has also been implicated to be involved in other steroid receptor pathways, including the retinoid pathway. RIP 140 has recently been shown to interact with peroxisome proliferator-activated receptor-alpha (PPAR alpha) and has been proposed to competitively inhibit PPAR alpha interaction with Steroid Receptor Coactivator-1 (SRC-1).
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab3425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 129 kDa).Can be blocked with RIP140 peptide (ab4968).|
FunctionModulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors.
DomainContains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors. The C-terminal LTKTNPILYYMLQK motif is required for ligand-dependent interaction with RAAR and RXRB homodimers and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB (By similarity). Contains at least four autonomous repression domains (RD1-4). RD1 functions via a histone deacetylase (HDAC)-independent mechanism, whereas RD2, RD3 and RD4 can function by HDAC-dependent or independent mechanisms, depending on cell type. RD2 is dependent on CTBP binding.
modificationsAcetylation regulates its nuclear translocation and corepressive activity (By similarity). Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH.
Cellular localizationNucleus. Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1.
- Information by UniProt
- NRIP 1 antibody
- NRIP1 antibody
- NRIP1_HUMAN antibody
This product has been referenced in:
- Al-Rewashdy H et al. Utrophin A is essential in mediating the functional adaptations of mdx mouse muscle following chronic AMPK activation. Hum Mol Genet 24:1243-55 (2015). Read more (PubMed: 25324540) »
- Gidlund EK et al. Rapidly elevated levels of PGC-1a-b protein in human skeletal muscle after exercise: exploring regulatory factors in a randomized controlled trial. J Appl Physiol (1985) 119:374-84 (2015). Read more (PubMed: 26089547) »