Overview

  • Product name

  • Description

    Rabbit polyclonal to RIP140
  • Host species

    Rabbit
  • Specificity

    ab42125 recognises RIP140 at the N terminal region containing the HDAC binding domain.
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) corresponding to the N terminal domain of RIP140.

Properties

Applications

Our Abpromise guarantee covers the use of ab42125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/200.
WB 1/1000. Predicted molecular weight: 129 kDa.

Target

  • Function

    Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors.
  • Domain

    Contains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors. The C-terminal LTKTNPILYYMLQK motif is required for ligand-dependent interaction with RAAR and RXRB homodimers and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB (By similarity). Contains at least four autonomous repression domains (RD1-4). RD1 functions via a histone deacetylase (HDAC)-independent mechanism, whereas RD2, RD3 and RD4 can function by HDAC-dependent or independent mechanisms, depending on cell type. RD2 is dependent on CTBP binding.
  • Post-translational
    modifications

    Acetylation regulates its nuclear translocation and corepressive activity (By similarity). Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH.
  • Cellular localization

    Nucleus. Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1.
  • Information by UniProt
  • Database links

  • Alternative names

    • NRIP 1 antibody
    • NRIP1 antibody
    • NRIP1_HUMAN antibody
    • Nuclear factor RIP 140 antibody
    • Nuclear factor RIP140 antibody
    • Nuclear receptor interacting protein 1 antibody
    • Nuclear receptor-interacting protein 1 antibody
    • Receptor interacting protein 140 antibody
    • Receptor-interacting protein 140 antibody
    • RIP 140 antibody
    • RIP140 antibody
    see all

References

This product has been referenced in:

  • Ding Z  et al. Interaction with AEG-1 and MDM2 is associated with glioma development and progression and correlates with poor prognosis. Cell Cycle N/A:N/A (2018). Read more (PubMed: 30560724) »
  • Vivante A  et al. A Dominant Mutation in Nuclear Receptor Interacting Protein 1 Causes Urinary Tract Malformations via Dysregulation of Retinoic Acid Signaling. J Am Soc Nephrol 28:2364-2376 (2017). Read more (PubMed: 28381549) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - nuclear (MCF7)
Loading amount
50 µg
Specification
MCF7
Gel Running Conditions
Non-reduced Denaturing (8% Acrylamide)
Blocking step
Milk as blocking agent for 25 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 01 2011

Application
Western blot
Sample
Monkey Cell lysate - nuclear (COS7)
Loading amount
50 µg
Specification
COS7
Gel Running Conditions
Non-reduced Denaturing (6% Acrylamide)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 30 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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