• Product name

  • Description

    Rabbit polyclonal to RIP2
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 11/30 of Human RIP2. (Peptide available as ab8463.)

  • Positive control

    • A431 or K562 whole cell lysate An approximately60 kDa band can be detected.


Our Abpromise guarantee covers the use of ab8428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 60 kDa.
ICC/IF Use a concentration of 10 µg/ml.


  • Function

    Activates pro-caspase-1 and pro-caspase-8. Potentiates CASP8-mediated apoptosis. Activates NF-kappa-B.
  • Tissue specificity

    Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 CARD domain.
    Contains 1 protein kinase domain.
  • Post-translational

    Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination catalyzed by ITCH.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • CARD 3 antibody
    • CARD carrying kinase antibody
    • CARD containing ICE associated kinase antibody
    • CARD containing IL 1 beta ICE kinase antibody
    • CARD containing IL1 beta ICE kinase antibody
    • CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase antibody
    • CARD containing interleukin 1 beta converting enzyme associated kinase antibody
    • CARD-containing IL-1 beta ICE-kinase antibody
    • CARD-containing interleukin-1 beta-converting enzyme-associated kinase antibody
    • CARD3 antibody
    • CARDIAK antibody
    • CCK antibody
    • CLARP kinase antibody
    • GIG 30 antibody
    • GIG30 antibody
    • Growth inhibiting gene 30 antibody
    • Receptor interacting protein (RIP) like interacting caspase like apoptosis regulatory protein (CLARP) kinase antibody
    • Receptor interacting protein 2 antibody
    • Receptor interacting serine threonine kinase 2 antibody
    • Receptor interacting serine/threonine protein kinase 2 antibody
    • Receptor-interacting protein 2 antibody
    • Receptor-interacting serine/threonine-protein kinase 2 antibody
    • RICK antibody
    • RIP 2 antibody
    • RIP like interacting CLARP kinase antibody
    • RIP-2 antibody
    • RIP-like-interacting CLARP kinase antibody
    • RIPK 2 antibody
    • Ripk2 antibody
    • RIPK2_HUMAN antibody
    • TNFRSF antibody
    • Tyrosine-protein kinase RIPK2 antibody
    • UNQ277/PRO314/PRO34092 antibody
    see all


  • Immunocytochemistry/ Immunofluorescence of K562 cells staining RIP2 with ab8428 at 20µg/ml
  • Western Blot of HeLa (1), Ramos (2) and EL4 (3) cell lysates labeling RIP2 with Anti-RIP2 antibody (ab8428) at 1µg/ml.
  • All lanes : Anti-RIP2 antibody (ab8428) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : K562 whole cell lysate

    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

  • Ab8428 at 20μg/ml staining RIP2 in K562 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). 

  • ab8428 at 10µg/ml staining RIP2 in A431 cells by ICC/IF 


This product has been referenced in:

  • Wang H  et al. Rip2 Is Required for Nod2-Mediated Lysozyme Sorting in Paneth Cells. J Immunol 198:3729-3736 (2017). Read more (PubMed: 28330897) »
  • Emmerich CH  et al. Lys63/Met1-hybrid ubiquitin chains are commonly formed during the activation of innate immune signalling. Biochem Biophys Res Commun 474:452-461 (2016). Read more (PubMed: 27133719) »
See all 3 Publications for this product

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