Product nameAnti-RIP2 antibody [AF28D3]
See all RIP2 primary antibodies
DescriptionMouse monoclonal [AF28D3] to RIP2
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human RIP2.
- HeLa, 293T, Ramos, C6 and L929 cell lysates.
This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR3185124 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.03% Sodium azide
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, HEPES
PurityTissue culture supernatant
Light chain typekappa
Our Abpromise guarantee covers the use of ab75257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa.|
FunctionActivates pro-caspase-1 and pro-caspase-8. Potentiates CASP8-mediated apoptosis. Activates NF-kappa-B.
Tissue specificityDetected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 CARD domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination catalyzed by ITCH.
- Information by UniProt
- CARD 3 antibody
- CARD carrying kinase antibody
- CARD containing ICE associated kinase antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: RIP2 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Ramos cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab75257 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab75257 was shown to recognize RIP2 when RIP2 knockout samples were used, along with additional cross-reactive bands. Wild-type and RIP2 knockout samples were subjected to SDS-PAGE. ab75257 and ab181602 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
All lanes : Anti-RIP2 antibody [AF28D3] (ab75257) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : C6 cell lysate
Lane 5 : L929 cell lysate
Predicted band size: 61 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
ab75257 has not yet been referenced specifically in any publications.