Product nameRIP2 overexpression 293T lysate (whole cell)
See all RIP2 lysates
DescriptionRIP2 overexpression lysate
ab94131 is a 293T cell transfected lysate in which Human RIP2 has been transiently over-expressed using a pCMV-RIP2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
Concentration information loading...
BackgroundFunction: Activates pro-caspase-1 and pro-caspase-8. Potentiates CASP8-mediated apoptosis. Activates NF-kappa-B. Tissue specificity: Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node. Similarity: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 1 CARD domain. Contains 1 protein kinase domain. PTM: Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR. Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination catalyzed by ITCH.
Our Abpromise guarantee covers the use of ab94131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94131 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-RIP2 antibody (ab57952) at 1/500 dilution
Lane 1 :
RIP2 overexpression 293T lysate (whole cell) (ab94131)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94131 has not yet been referenced specifically in any publications.