Anti-RIP3 antibody (ab226297)
Key features and details
- Rabbit polyclonal to RIP3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RIP3 antibody
See all RIP3 primary antibodies -
Description
Rabbit polyclonal to RIP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide within Human RIP3 aa 400-450. The exact sequence is proprietary. (NP_006862.2).
Database link: Q9Y572 -
Positive control
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab226297 was affinity purified using an epitope specific to RIP3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 57 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 57 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Promotes apoptosis. -
Tissue specificity
Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 452820 Chimpanzee
- Entrez Gene: 101126247 Gorilla
- Entrez Gene: 11035 Human
- Entrez Gene: 100461174 Orangutan
- Omim: 605817 Human
- SwissProt: Q9Y572 Human
- Unigene: 268551 Human
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Alternative names
- Receptor interacting protein 3 antibody
- Receptor interacting serine threonine kinase 3 antibody
- Receptor interacting serine/threonine protein kinase 3 antibody
see all
Images
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All lanes : Anti-RIP3 antibody (ab226297) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate prepared using NETN buffer
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate prepared using NETN buffer
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate prepared using NETN buffer
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate prepared using RIPA buffer
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 30 seconds -
RIP3 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using RIPA buffer; 1 mg for IP, 20% of IP loaded) with ab226297 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226297 at 1 µg/ml.
Lane 1: ab226297 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab226297 has been referenced in 1 publication.
- Guo R et al. Activation of Delta-Opioid Receptor Protects ARPE19 Cells against Oxygen-Glucose Deprivation/Reoxygenation-Induced Necroptosis and Apoptosis by Inhibiting the Release of TNF-α. J Ophthalmol 2022:2285663 (2022). PubMed: 36457949