Overview

  • Product name

  • Description

    Rabbit polyclonal to RIP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human RIP3 aa 400-450. The exact sequence is proprietary. (NP_006862.2).
    Database link: Q9Y572

  • Positive control

    • WB: Jurkat whole cell lysate (ab7899). IP: Jurkat whole cell lysate (ab7899).

Properties

Applications

Our Abpromise guarantee covers the use of ab226297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 57 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Promotes apoptosis.
  • Tissue specificity

    Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Receptor interacting protein 3 antibody
    • Receptor interacting serine threonine kinase 3 antibody
    • Receptor interacting serine/threonine protein kinase 3 antibody
    • Receptor-interacting protein 3 antibody
    • Receptor-interacting serine/threonine-protein kinase 3 antibody
    • RIP 3 antibody
    • RIP like protein kinase 3 antibody
    • RIP-3 antibody
    • RIP-like protein kinase 3 antibody
    • RIPK 3 antibody
    • RIPK3 antibody
    • RIPK3_HUMAN antibody
    see all

Images

  • All lanes : Anti-RIP3 antibody (ab226297) at 0.4 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate prepared using NETN buffer
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate prepared using NETN buffer
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate prepared using NETN buffer
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate prepared using RIPA buffer

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 57 kDa


    Exposure time: 30 seconds
  • RIP3 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using RIPA buffer; 1 mg for IP, 20% of IP loaded) with ab226297 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226297 at 1 µg/ml.

    Lane 1: ab226297 IP in Jurkat whole cell lysate.

    Lane 2: Control IgG IP in Jurkat whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 30 seconds.

References

ab226297 has not yet been referenced specifically in any publications.

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