Product nameAnti-RIP3 (phospho S227) antibody [EPR9627]
See all RIP3 primary antibodies
DescriptionRabbit monoclonal [EPR9627] to RIP3 (phospho S227)
Tested applicationsSuitable for: ELISA, Dot blot, WBmore details
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RIP3 aa 200-300 (phospho S227). The exact sequence is proprietary.
Database link: Q9Y572
- WB: HT-29 treated with 20 ng/ml TNF alpha,100 nM Smac mimetic, and 20 mM z-VAD for 8 h and then harvested whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab209384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).|
Tissue specificityDetected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
- Information by UniProt
- Receptor interacting protein 3 antibody
- Receptor interacting serine threonine kinase 3 antibody
- Receptor interacting serine/threonine protein kinase 3 antibody
All lanes : Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)
Lane 1 : Untreated HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2 : HT-29 (Human colorectal adenocarcinoma cell line) treated with 20 ng/ml TNF alpha,100 nM Smac mimetic, and 20 uM z-VAD for 6 h and then harvested whole cell lysate
Predicted band size: 57 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Blocking/Dilution buffer: 5% NFDM/TBST.
Image provided by Xiaodong. Wang, National Institute of Biological Sciences, Beijing.
Dot blot analysis of RIP3 (phospho S227) phospho peptide (Lane 1) and RIP3 non-phospho peptide (Lane 2) labeled using ab209384 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
Direct ELISA antibody dose-response curve using ab209384. Antigen concentration of 1000 ng/mL and primary antibody concentration of 0-1000 ng/mL. A Alkaline Phosphatase-conjugated AffiniPure -conjugated goat anti-rabbit IgG (1/2500) was used as the secondary antibody.
This product has been referenced in:
- Ali M & Mocarski ES Proteasome inhibition blocks necroptosis by attenuating death complex aggregation. Cell Death Dis 9:346 (2018). Read more (PubMed: 29497034) »
- Sureshbabu A et al. RIPK3 promotes sepsis-induced acute kidney injury via mitochondrial dysfunction. JCI Insight 3:N/A (2018). Read more (PubMed: 29875323) »