Overview

  • Product name

    Anti-RIP3 (phospho T231 + S232) antibody [2D7]
    See all RIP3 primary antibodies
  • Description

    Mouse monoclonal [2D7] to RIP3 (phospho T231 + S232)
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse RIP3 (phospho T231 + S232).

  • Positive control

    • WB: Human and mouse pancreas tissue lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab205421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

Target

  • Function

    Promotes apoptosis.
  • Tissue specificity

    Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Receptor interacting protein 3 antibody
    • Receptor interacting serine threonine kinase 3 antibody
    • Receptor interacting serine/threonine protein kinase 3 antibody
    • Receptor-interacting protein 3 antibody
    • Receptor-interacting serine/threonine-protein kinase 3 antibody
    • RIP 3 antibody
    • RIP like protein kinase 3 antibody
    • RIP-3 antibody
    • RIP-like protein kinase 3 antibody
    • RIPK 3 antibody
    • RIPK3 antibody
    • RIPK3_HUMAN antibody
    see all

Images

  • All lanes : Anti-RIP3 (phospho T231 + S232) antibody [2D7] (ab205421) at 1 µg/ml

    Lane 1 : Human pancreas tissue lysate - total protein (ab29816) at 10 µg
    Lane 2 : Pancreas (Mouse) Tissue Lysate at 10 µg
    Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
    Lane 4 : Human pancreas tissue lysate - total protein (ab29816) at 10 µg with RIP3 Peptide (non-mod) at 1 µg/ml
    Lane 5 : Pancreas (Mouse) Tissue Lysate at 10 µg with RIP3 peptide (non-mod) at 1 µg/ml
    Lane 6 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg with RIP3 Peptide (non-mod) at 1 µg/ml
    Lane 7 : Human pancreas tissue lysate - total protein (ab29816) with RIP3 phospho T231 + S232 peptide at 1 µg/ml
    Lane 8 : Pancreas (Mouse) Tissue Lysate at 10 µg with RIP3 phospho T231 + S232 peptide at 1 µg/ml
    Lane 9 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg with RIP3 phospho T231 + S232 peptide at 1 µg/ml

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 57 kDa


    Exposure time: 30 seconds
  • Serially diluted ab205421 was bound to immobilised RIP3 phospho peptide (T231), RIP3 phospho peptide (S232), RIP3 dual phospho peptide (T231+S232) or RIP3 control peptide (RIP3-nP; all peptides at 1 microgram x mL-1). The antibody was detected by HRP-labeled goat anti-mouse IgG and signal was developed with TMB substrate.

References

This product has been referenced in:

See all 4 Publications for this product

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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