Anti-RIPX antibody (ab229155)
Key features and details
- Rabbit polyclonal to RIPX
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RIPX antibody
See all RIPX primary antibodies -
Description
Rabbit polyclonal to RIPX -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Xenopus laevis -
Immunogen
Recombinant fragment within Human RIPX (internal sequence). The exact sequence is proprietary.
Database link: Q7L099 -
Positive control
- WB: IMR32 whole cell lysate; Mouse brain tissue lysate. IHC-P: U251 xenograft tissue; Mouse lung tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. |
Target
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Function
Implicated in the formation of a single axon by developing neurons. May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes. -
Sequence similarities
Contains 1 RUN domain. -
Post-translational
modificationsIsoform 1 is partially phosphorylated. -
Cellular localization
Cell projection. Enriched in F-actin enriched filopodia and lamellipodia of growth cones of axons and minor processes of hippocampal neurons in culture. - Information by UniProt
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Database links
- Entrez Gene: 22902 Human
- Entrez Gene: 52822 Mouse
- Entrez Gene: 360921 Rat
- GenBank: NM_014961 Human
- Omim: 611194 Human
- SwissProt: Q7L099 Human
- SwissProt: Q9D394 Mouse
- SwissProt: Q5FVJ0 Rat
see all -
Alternative names
- Protein RUFY3 antibody
- rap2 interacting protein x antibody
- Rap2 interacting protein x isoform 2 antibody
see all
Images
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Anti-RIPX antibody (ab229155) at 1/3000 dilution + IMR32 whole cell lysate at 30 µg
7.5% SDS-PAGE gel.
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Anti-RIPX antibody (ab229155) at 1/1000 dilution + Mouse brain tissue lysate at 50 µg
10% SDS-PAGE gel.
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Paraffin-embedded U251 xenograft tissue stained for RIPX using ab229155 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse lung tissue stained for RIPX using ab229155 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229155 has not yet been referenced specifically in any publications.