Overview

  • Product name

  • Description

    Rabbit Polyclonal to RIPX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human RIPX aa 243-352.
    Database link: Q7L099

  • Positive control

    • WB: HeLa, HEK-293T, K562 and SH-SY5Y whole cell lysates. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity greater than 95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab237511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 53 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Implicated in the formation of a single axon by developing neurons. May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes.
  • Sequence similarities

    Contains 1 RUN domain.
  • Post-translational
    modifications

    Isoform 1 is partially phosphorylated.
  • Cellular localization

    Cell projection. Enriched in F-actin enriched filopodia and lamellipodia of growth cones of axons and minor processes of hippocampal neurons in culture.
  • Information by UniProt
  • Database links

  • Alternative names

    • Protein RUFY3 antibody
    • rap2 interacting protein x antibody
    • Rap2 interacting protein x isoform 2 antibody
    • Rap2-interacting protein x antibody
    • RIPx antibody
    • Rpipx antibody
    • Rufy3 antibody
    • RUFY3_HUMAN antibody
    • RUN and FYVE domain containing 3 antibody
    • Singar antibody
    • Singar1 antibody
    • Single axon regulated protein antibody
    • single axon related 1 antibody
    • Single axon-regulated protein antibody
    see all

Images

  • 4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RIPX (green) using ab237511 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.

  • All lanes : Anti-RIPX antibody (ab237511) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 53 kDa

References

ab237511 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab237511.
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