Overview

  • Product name

  • Description

    Mouse polyclonal to RIPX
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Full length Human RIPX (NP_055776.1).

  • Positive control

    • Rat brain tissue lysate and RIPX transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab89147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa.

Target

  • Function

    Implicated in the formation of a single axon by developing neurons. May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes.
  • Sequence similarities

    Contains 1 RUN domain.
  • Post-translational
    modifications

    Isoform 1 is partially phosphorylated.
  • Cellular localization

    Cell projection. Enriched in F-actin enriched filopodia and lamellipodia of growth cones of axons and minor processes of hippocampal neurons in culture.
  • Information by UniProt
  • Database links

  • Alternative names

    • Protein RUFY3 antibody
    • rap2 interacting protein x antibody
    • Rap2 interacting protein x isoform 2 antibody
    • Rap2-interacting protein x antibody
    • RIPx antibody
    • Rpipx antibody
    • Rufy3 antibody
    • RUFY3_HUMAN antibody
    • RUN and FYVE domain containing 3 antibody
    • Singar antibody
    • Singar1 antibody
    • Single axon regulated protein antibody
    • single axon related 1 antibody
    • Single axon-regulated protein antibody
    see all

Images

  • Anti-RIPX antibody (ab89147) at 1 µg/ml + Rat brain tissue lysate at 50 µg

    Predicted band size: 53 kDa
    Observed band size: 49 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-RIPX antibody (ab89147) at 1 µg/ml

    Lane 1 : RIPX transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

See 1 Publication for this product

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