Overview

  • Product name

  • Description

    Rabbit polyclonal to RIT1/RIT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human RIT1/RIT.

  • Positive control

    • Jurkat cells
  • General notes

     This product was previously labelled as RIT1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab53720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/10000.
WB 1/500 - 1/1000. Predicted molecular weight: 25 kDa.

Target

  • Function

    Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation.
  • Tissue specificity

    Expressed in many tissues.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Ras family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • GTP binding protein Roc1 antibody
    • GTP-binding protein Rit1 antibody
    • NS8 antibody
    • Ras like protein expressed in many tissues antibody
    • Ras like without CAAX 1 antibody
    • Ras-like protein expressed in many tissues antibody
    • Ras-like without CAAX protein 1 antibody
    • RIBB antibody
    • Ric like expressed in many tissues antibody
    • Ric-like protein without CAAX motif 1 antibody
    • RIT antibody
    • RIT1 antibody
    • RIT1_HUMAN antibody
    • ROC1 antibody
    see all

Images

  • ICC/IF image of ab53720 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53720, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab53720 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Thank you for contacting us. The immunogen is from the C terminus. Please let me know if you have any further questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up