Overview

  • Product name

    Rituximab ELISA Kit (Mabthera®)
    See all Rituximab kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 15%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 15%
  • Sample type

    Serum, Plasma
  • Assay type

    Quantitative
  • Sensitivity

    3 ng/ml
  • Range

    3 ng/ml - 300 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 85% - 115%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Rituximab ELISA Kit (Mabthera®) (ab237640) is a highly specific and sensitive kit designed for the in vitro determination of Rituximab in biological matrices such as human serum and plasma. The density of color is proportional to the amount of Rituximab captured from the samples and can be quantified when compared with standard curve.


    Rituximab (Mabthera®) is a genetically engineered chimeric murine/human monoclonal antibody specific to CD20. CD20 is an approximately 35 KDa transmembrane phosphoprotein involved in the activation, proliferation and differentiation of B-lymphocytes. It is absent in terminally differentiated plasma cells. The Fab domain of Rituximab binds to the CD20 antigen on B-lymphocytes and the Fc domain recruits immune effector functions to induce apoptosis in B cells and is used in the treatment of leukemias and lymphomas, some autoimmune disorders and organ transplant.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 96 tests
    Assay Buffer 1 x 50ml
    HRP-conjugate Probe 1 x 12ml
    Micro ELISA Plate 1 unit
    Plate sealers 2 units
    Rituximab Standard S1 1 x 1ml
    Rituximab Standard S2 1 x 1ml
    Rituximab Standard S3 1 x 1ml
    Rituximab Standard S4 1 x 1ml
    Rituximab Standard S5 1 x 1ml
    Rituximab Standard S6 1 x 1ml
    Rituximab Standard S7 1 x 1ml
    Rituximab Standard S8 1 x 1ml
    Stop Solution 1 x 12ml
    TMB substrate 1 x 12ml
    Wash buffer (20X) 1 x 50ml
  • Research areas

  • Relevance

    Rituximab is a genetically engineered chimeric murine/human monoclonal antibody specific to CD20. CD20 is an approximately 35 KDa transmembrane phosphoprotein involved in the activation, proliferation, and differentiation of B-lymphocytes. It is absent in terminally differentiated plasma cells. The Fab domain of Rituximab binds to the CD20 antigen on B-lymphocytes and the Fc domain recruits immune effector functions to induce apoptosis in B cells and is used in the treatment of leukemia s and lymphomas, some autoimmune disorders, and organ transplant.
  • Cellular localization

    Membrane; Multipass membrane protein.
  • Alternative names

    • B lymphocyte antigen CD20
    • B lymphocyte surface antigen B1
    • Bp35
    • CD20
    • Leu 16
    • Leu16
    • Membrane spanning 4 domains subfamily A member 1
    • MS4A1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab237640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.

Protocols

References

ab237640 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab237640.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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