Overview

  • Product name
  • Description
    Goat polyclonal to RKIP
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human RKIP aa 174-186 (C terminal).
    Sequence:

    C-DYVPKLYEQLSGK


    (Peptide available as ab22921)

  • Positive control
    • WB: A549 and HepG2 cells lysates, human liver tissue lysate. IHC-P: Human brain (cortex) tissue.
  • General notes
    GenBank Accession Number – NP_002558.

Properties

Applications

Our Abpromise guarantee covers the use of ab2634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 20-22 kDa (predicted molecular weight: 22 kDa).Can be blocked with Human RKIP peptide (ab22921).

Target

  • Function
    Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
    HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.
  • Sequence similarities
    Belongs to the phosphatidylethanolamine-binding protein family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis luminal protein 210 antibody
    • Epididymis secretory protein Li 34 antibody
    • Epididymis secretory protein Li 96 antibody
    • HCNP antibody
    • HCNPpp antibody
    • HEL 210 antibody
    • HEL S 34 antibody
    • HEL S 96 antibody
    • Hippocampal cholinergic neurostimulating peptide antibody
    • Neuropolypeptide h3 antibody
    • PBP antibody
    • PEBP antibody
    • PEBP 1 antibody
    • PEBP-1 antibody
    • Pebp1 antibody
    • PEBP1_HUMAN antibody
    • Phosphatidylethanolamine binding protein antibody
    • Phosphatidylethanolamine binding protein 1 antibody
    • Prostatic binding protein antibody
    • Prostatic-binding protein antibody
    • R kip antibody
    • Raf kinase inhibitor protein antibody
    • Raf kinase inhibitory protein antibody
    • RKIP antibody
    see all

Images

  • Anti-RKIP antibody (ab2634) at 1 µg/ml + A549 lysate at 35 µg

    Predicted band size: 22 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cortex tissue labelling RKIP with ab2634 at 5µg/ml. Heat induced antigen retrieval was performed with citrate buffer pH 6. HRP-staining.

  • Negative control Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cortex tissue using no primary antibody.

References

This product has been referenced in:
  • Chen Y  et al. The expression, induction and pharmacological activity of CYP1A2 are post-transcriptionally regulated by microRNA hsa-miR-132-5p. Biochem Pharmacol 145:178-191 (2017). WB . Read more (PubMed: 28822783) »
  • Fougner SL  et al. Low levels of raf kinase inhibitory protein in growth hormone-secreting pituitary adenomas correlate with poor response to octreotide treatment. J Clin Endocrinol Metab 93:1211-6 (2008). WB ; Human . Read more (PubMed: 18230656) »
See all 3 Publications for this product

Customer reviews and Q&As

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Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Hippocampus)
Loading amount
50 µg
Specification
Hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Dr. Martin Maurer

Verified customer

Submitted Oct 31 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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