Recombinant Anti-RKIP antibody [EPR2875Y] (ab76582)
- Datasheet
- References (10)
- Protocols
Overview
-
Product nameAnti-RKIP antibody [EPR2875Y]
See all RKIP primary antibodies -
DescriptionRabbit monoclonal [EPR2875Y] to RKIP
-
Host speciesRabbit
-
Tested applicationsSuitable for: Flow Cyt, IHC-P, IHC-Fr, WB, IP, ICC/IFmore details
-
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide within Human RKIP aa 50-150. The exact sequence is proprietary.
-
Positive control
- WB: HeLa, A549, HepG2 and A431 cell lysate. IHC-P: Human brain tissue. Flow Cyt: HeLa cells.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR2875Y
-
IsotypeIgG
-
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab76582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/200. | |
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 22542739 | |
| WB | 1/1000 - 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). | |
| IP | 1/10. | |
| ICC/IF | 1/100. |
Target
-
FunctionBinds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor. -
Sequence similaritiesBelongs to the phosphatidylethanolamine-binding protein family.
-
Cellular localizationCytoplasm.
- Information by UniProt
-
Database links
- Entrez Gene: 5037 Human
- Entrez Gene: 23980 Mouse
- Entrez Gene: 29542 Rat
- Omim: 604591 Human
- SwissProt: P30086 Human
- SwissProt: P70296 Mouse
- SwissProt: P31044 Rat
- Unigene: 433863 Human
see all -
Alternative names
- Epididymis luminal protein 210 antibody
- Epididymis secretory protein Li 34 antibody
- Epididymis secretory protein Li 96 antibody
see all
Images
-
![Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)](https://www.abcam.com/ps/products/76/ab76582/Images/ab76582wb.gif)
Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)All lanes : Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)](https://www.abcam.com/ps/products/76/ab76582/Images/ab76582ihc.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)Staining of formalin-fixed, paraffin-embedded human brain tissue using 1/250 ab76582. -
![Flow Cytometry - Anti-RKIP antibody [EPR2875Y] (ab76582)](https://www.abcam.com/ps/products/76/ab76582/Images/ab76582-5-ab76582FC1.jpg)
Flow Cytometry - Anti-RKIP antibody [EPR2875Y] (ab76582)Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling RKIP (red) with ab76582 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
-
![Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)](https://www.abcam.com/ps/products/76/ab76582/Images/PBP-Primary-antibodies-ab76582-2.jpg)
Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/5000 dilution +Recombinant Human RKIP protein (ab87892) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Exposure time: 30 seconds
Protocols
Datasheets and documents
References
This product has been referenced in:
- Xie SY et al. RKIP reduction enhances radioresistance by activating the Shh signaling pathway in non-small-cell lung cancer. Onco Targets Ther 10:5605-5619 (2017). Read more (PubMed: 29200875) »
- Wang Z et al. Phosphorylation at S153 as a Functional Switch of Phosphatidylethanolamine Binding Protein 1 in Cerebral Ischemia-Reperfusion Injury in Rats. Front Mol Neurosci 10:358 (2017). Read more (PubMed: 29163033) »
