Name: Anti-RKIP antibody [EPR2875Y]
Brand: RabMAb
SKU: ab76582
Rating: 5 (5 reviews)

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Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2875Y] to RKIP
Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
Reacts with: Mouse, Rat, Human

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Primary

Protein

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Primary - Rabbit Anti-RKIP antibody [EPR2875Y] (ab76582)
Flow Cyt, IHC-P, WB, ICC/IF

Protein - Recombinant Human RKIP protein (ab87892)
SDS-PAGE, WB

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Product name

Anti-RKIP antibody [EPR2875Y]
See all RKIP primary antibodies
Description

Rabbit monoclonal [EPR2875Y] to RKIP
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human RKIP aa 50-150. The exact sequence is proprietary.
Positive control
- WB: HeLa, A549, HepG2, A431, mouse brain, mouse testis and rat brain cell lysates; IHC-P: human ovarian carcinoma and brain, mouse kidney, and rat testis tissues; ICC/IF: HepG2 cells; Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2875Y
Isotype

IgG
Research areas

Alternative Versions
- Anti-RKIP antibody [EPR2875Y] - BSA and Azide free (ab239887)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HepG2 cytoplasmic extract lysate (ab14659)
Recombinant Protein
- Recombinant Human RKIP protein (ab87892)

Our Abpromise guarantee covers the use of ab76582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt		1/20.
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
WB		1/1000 - 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ICC/IF		1/50 - 1/100.

Application notes

Is unsuitable for IP.

Function

Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.
Sequence similarities

Belongs to the phosphatidylethanolamine-binding protein family.
Cellular localization

Cytoplasm.
Target information above from: UniProt accession P30086 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 5037 Human
- Entrez Gene: 23980 Mouse
- Entrez Gene: 29542 Rat
- Omim: 604591 Human
- SwissProt: P30086 Human
- SwissProt: P70296 Mouse
- SwissProt: P31044 Rat
- Unigene: 433863 Human
- Unigene: 195898 Mouse
- Unigene: 338476 Mouse
- Unigene: 473809 Mouse
- Unigene: 29745 Rat
see all
Alternative names
- Epididymis luminal protein 210 antibody
- Epididymis secretory protein Li 34 antibody
- Epididymis secretory protein Li 96 antibody
- HCNP antibody
- HCNPpp antibody
- HEL 210 antibody
- HEL S 34 antibody
- HEL S 96 antibody
- Hippocampal cholinergic neurostimulating peptide antibody
- Neuropolypeptide h3 antibody
- PBP antibody
- PEBP antibody
- PEBP 1 antibody
- PEBP-1 antibody
- Pebp1 antibody
- PEBP1_HUMAN antibody
- Phosphatidylethanolamine binding protein antibody
- Phosphatidylethanolamine binding protein 1 antibody
- Prostatic binding protein antibody
- Prostatic-binding protein antibody
- R kip antibody
- Raf kinase inhibitor protein antibody
- Raf kinase inhibitory protein antibody
- RKIP antibody
see all

Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)

All lanes : Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/1000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : Mouse testis lysates
Lane 5 : Rat brain lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 21 kDa
Observed band size: 21 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RKIP antibody [EPR2875Y] (ab76582)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling RKIP with purified ab76582 at 1:50 dilution (2.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue sections labeling RKIP with purified ab76582 at 1/500 dilution (0.26 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Flow Cytometry - Anti-RKIP antibody [EPR2875Y] (ab76582)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling RKIP (red) with purified ab76582 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling RKIP with purified ab76582 at 1/500 dilution (0.26 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling RKIP with purified ab76582 at 1/500 dilution (0.26 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)

All lanes : Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/5000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : A431 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 21 kDa
Observed band size: 21 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

Staining of formalin-fixed, paraffin-embedded human brain tissue using 1/250 ab76582 (unpurified).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)

Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/5000 dilution (unpurified) + Recombinant Human RKIP protein (ab87892) at 0.1 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 21 kDa

Exposure time: 30 seconds

Flow cytometry protocols
Immunohistochemistry protocols

Click here to view the general protocols

Datasheet

Publishing research using ab76582? Please let us know so that we can cite the reference in this datasheet.

ab76582 has been referenced in 11 publications.

Raquel-Cunha A et al. Current Status of Raf Kinase Inhibitor Protein (RKIP) in Lung Cancer: Behind RTK Signaling. Cells 8:N/A (2019). PubMed: 31083461
Xie SY et al. RKIP reduction enhances radioresistance by activating the Shh signaling pathway in non-small-cell lung cancer. Onco Targets Ther 10:5605-5619 (2017). PubMed: 29200875
Wang Z et al. Phosphorylation at S153 as a Functional Switch of Phosphatidylethanolamine Binding Protein 1 in Cerebral Ischemia-Reperfusion Injury in Rats. Front Mol Neurosci 10:358 (2017). PubMed: 29163033
Papale M et al. Urinary RKIP/p-RKIP is a potential diagnostic and prognostic marker of clear cell renal cell carcinoma. Oncotarget 8:40412-40424 (2017). PubMed: 28418894
Su L et al. Raf kinase inhibitor protein regulates oxygen-glucose deprivation-induced PC12 cells apoptosis through the NF-?B and ERK pathways. J Clin Biochem Nutr 59:86-92 (2016). WB, IP . PubMed: 27698534

View all Publications for this product

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Application

Western blot

Sample

Human Cell lysate - whole cell (A2058 melanoma cell line)

Gel Running Conditions

Reduced Denaturing (12% Acrisbys Gel)

Loading amount

20 µg

Specification

A2058 melanoma cell line

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Cristina Penas Lago

Verified customer

Submitted Mar 06 2018

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (neuronal)

Specification

neuronal

Fixative

Formaldehyde

Permeabilization

Yes - a. PBS + 0.2% Triton x-100

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Aug 31 2012

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (NEURONAL)

Specification

NEURONAL

Fixative

Formaldehyde

Permeabilization

Yes - a. PBS + 0.2% Triton x-100

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Aug 30 2012

Application

Western blot

Sample

Mouse Cell lysate - whole cell (neuronal)

Loading amount

50 µg

Specification

neuronal

Gel Running Conditions

Non-reduced Denaturing

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Aug 30 2012

Application

Western blot

Sample

Human Cell lysate - whole cell (neuronal)

Loading amount

50 µg

Specification

neuronal

Gel Running Conditions

Non-reduced Denaturing

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Aug 30 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

Applications

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As

Western blot abreview for Anti-PBP antibody [EPR2875Y]

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Western blot abreview for Anti-PBP antibody [EPR2875Y]

Western blot abreview for Anti-PBP antibody [EPR2875Y]