Product nameAnti-RKIP (phospho S153) antibody [EP2845Y]
See all RKIP primary antibodies
DescriptionRabbit monoclonal [EP2845Y] to RKIP (phospho S153)
Specificityab75971 detects RKIP phosphorylated on serine 153.
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Rat, Human
corresponding to Human RKIP (phospho S153).
- HeLa cell lysates treated with calyculin A. Human liver tissue.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab75971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||1/100 - 1/250.|
FunctionBinds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.
Sequence similaritiesBelongs to the phosphatidylethanolamine-binding protein family.
- Information by UniProt
- Epididymis luminal protein 210 antibody
- Epididymis secretory protein Li 34 antibody
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All lanes : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/2000 dilution
Lane 1 : HeLa cell lysates un-treated
Lane 2 : HeLa cell lysates treated with calyculin A
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
ab75971, at 1/100 dilution, staining RKIP in human liver by immunohistochemistry using paraffin-embedded tissue.
This product has been referenced in:
- Wang Z et al. Phosphorylation at S153 as a Functional Switch of Phosphatidylethanolamine Binding Protein 1 in Cerebral Ischemia-Reperfusion Injury in Rats. Front Mol Neurosci 10:358 (2017). Read more (PubMed: 29163033) »
- Macrae K et al. Defining the role of DAG, mitochondrial function, and lipid deposition in palmitate-induced proinflammatory signaling and its counter-modulation by palmitoleate. J Lipid Res 54:2366-78 (2013). WB . Read more (PubMed: 23833248) »