Overview

  • Product name

    Anti-RMI2 antibody
  • Description

    Rabbit polyclonal to RMI2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: GKYVMVMGVV QACSPEPCLQ AVKMTDLSDN PIHESMWELE VEDLHRNI, corresponding to amino acids 99-146 of Human RMI2.

  • Positive control

    • Human stomach tissue; RT 4, U 251 MG and Human liver lysates; U 251 MG cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab122685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.
WB 1/250 - 1/500.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. The complex is therefore essential for the stability, localization, and function of complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM.
  • Sequence similarities

    Belongs to the RMI2 family.
    Contains 1 OB DNA-binding domain.
  • Post-translational
    modifications

    Phosphorylated during mitosis.
  • Cellular localization

    Nucleus. Colocalizes with BLM at nuclear DNA repair foci.
  • Information by UniProt
  • Database links

  • Alternative names

    • BLAP18 antibody
    • BLM-associated protein of 18 kDa antibody
    • C16orf75 antibody
    • hRMI2 antibody
    • MGC24665 antibody
    • RecQ-mediated genome instability protein 2 antibody
    • RMI2 antibody
    • RMI2_HUMAN antibody
    see all

Images

  • ab122685, at 1/200 dilution, staining RMI2 in paraffin-embedded Human stomach tissue by Immunohistochemistry.
  • ab122685, at 4 µg/ml staining RIM2 in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).
  • All lanes : Anti-RMI2 antibody (ab122685) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

References

This product has been referenced in:

  • Zhang W  et al. Targeting the MYCN-PARP-DNA Damage Response Pathway in Neuroendocrine Prostate Cancer. Clin Cancer Res 24:696-707 (2018). Read more (PubMed: 29138344) »
  • Nicholls TJ  et al. Topoisomerase 3a Is Required for Decatenation and Segregation of Human mtDNA. Mol Cell 69:9-23.e6 (2018). Read more (PubMed: 29290614) »
See all 2 Publications for this product

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