Product nameAnti-RMI2 antibody
DescriptionRabbit polyclonal to RMI2
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- Human stomach tissue; RT 4, U 251 MG and Human liver lysates; U 251 MG cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.|
|WB||1/250 - 1/500.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionEssential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. The complex is therefore essential for the stability, localization, and function of complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM.
Sequence similaritiesBelongs to the RMI2 family.
Contains 1 OB DNA-binding domain.
modificationsPhosphorylated during mitosis.
Cellular localizationNucleus. Colocalizes with BLM at nuclear DNA repair foci.
- Information by UniProt
- BLAP18 antibody
- BLM-associated protein of 18 kDa antibody
- C16orf75 antibody
ab122685, at 1/200 dilution, staining RMI2 in paraffin-embedded Human stomach tissue by Immunohistochemistry.
ab122685, at 4 µg/ml staining RIM2 in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).
All lanes : Anti-RMI2 antibody (ab122685) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
This product has been referenced in:
- Zhang W et al. Targeting the MYCN-PARP-DNA Damage Response Pathway in Neuroendocrine Prostate Cancer. Clin Cancer Res 24:696-707 (2018). Read more (PubMed: 29138344) »
- Nicholls TJ et al. Topoisomerase 3a Is Required for Decatenation and Segregation of Human mtDNA. Mol Cell 69:9-23.e6 (2018). Read more (PubMed: 29290614) »