Anti-RMND1 antibody (ab223119)
Key features and details
- Rabbit polyclonal to RMND1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RMND1 antibody -
Description
Rabbit polyclonal to RMND1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human RMND1 aa 35-160.
Database link: Q9NWS8 -
Positive control
- WB: MCF7 and HEK-293 cell lysates; Mouse skeletal muscle lysate. IHC-P: Human adrenal gland tissue and ovarian cancer tissues. ICC/IF: MCF7 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab223119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/50 - 1/200. | |
| WB | 1/500 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa). | |
| IHC-P | 1/20 - 1/200. |
Target
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Function
Required for mitochondrial translation, possibly by coordinating the assembly or maintenance of the mitochondrial ribosome (PubMed:23022098, PubMed:25604853). -
Involvement in disease
Combined oxidative phosphorylation deficiency 11 -
Sequence similarities
Belongs to the RMD1/sif2 family. -
Cellular localization
Mitochondrion. May be localized in mitochondrial RNA granules (PubMed:25604853). - Information by UniProt
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Database links
- Entrez Gene: 55005 Human
- Entrez Gene: 66084 Mouse
- SwissProt: Q9NWS8 Human
- SwissProt: Q8CI78 Mouse
- Unigene: 486835 Human
- Unigene: 744622 Human
- Unigene: 153111 Mouse
- Unigene: 313137 Mouse
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Alternative names
- bA351K16 antibody
- bA351K16.3 antibody
- C6orf96 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-RMND1 antibody (ab223119)MCF7 (human breast adenocarcinoma cell line) cells stained for RMND1 (green) using ab223119 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RMND1 antibody (ab223119)Paraffin-embedded human ovarian cancer tissue stained for RMND1 using ab223119 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RMND1 antibody (ab223119)Paraffin-embedded human adrenal gland tissue stained for RMND1 using ab223119 at 1/100 dilution in immunohistochemical analysis.
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Western blot - Anti-RMND1 antibody (ab223119)All lanes : Anti-RMND1 antibody (ab223119) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 3 : Mouse skeletal muscle lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 51 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References (0)
ab223119 has not yet been referenced specifically in any publications.
