Overview

  • Product name

    Anti-RMND1 antibody
  • Description

    Rabbit polyclonal to RMND1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human RMND1 aa 35-160.
    Database link: Q9NWS8

  • Positive control

    • WB: MCF7 and HEK-293 cell lysates; Mouse skeletal muscle lysate. IHC-P: Human adrenal gland tissue and ovarian cancer tissues. ICC/IF: MCF7 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
WB 1/500 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa).
IHC-P 1/20 - 1/200.

Target

  • Function

    Required for mitochondrial translation, possibly by coordinating the assembly or maintenance of the mitochondrial ribosome (PubMed:23022098, PubMed:25604853).
  • Involvement in disease

    Combined oxidative phosphorylation deficiency 11
  • Sequence similarities

    Belongs to the RMD1/sif2 family.
  • Cellular localization

    Mitochondrion. May be localized in mitochondrial RNA granules (PubMed:25604853).
  • Information by UniProt
  • Database links

  • Alternative names

    • bA351K16 antibody
    • bA351K16.3 antibody
    • C6orf96 antibody
    • chromosome 6 open reading frame 96 antibody
    • FLJ20627 antibody
    • MGC117362 antibody
    • MGC149570 antibody
    • MGC882602 antibody
    • required for meiotic nuclear division 1 homolog (S. cerevisiae) antibody
    • Required for meiotic nuclear division protein 1 homolog antibody
    • Rmnd1 antibody
    • RMND1_HUMAN antibody
    see all

Images

  • MCF7 (human breast adenocarcinoma cell line) cells stained for RMND1 (green) using ab223119 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • Paraffin-embedded human ovarian cancer tissue stained for RMND1 using ab223119 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human adrenal gland tissue stained for RMND1 using ab223119 at 1/100 dilution in immunohistochemical analysis.

  • All lanes : Anti-RMND1 antibody (ab223119) at 1/500 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 3 : Mouse skeletal muscle lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 51 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?

References

ab223119 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223119.
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