Anti-RMND1 antibody (ab223119)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RMND1 antibody -
Description
Rabbit polyclonal to RMND1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human RMND1 aa 35-160.
Database link: Q9NWS8 -
Positive control
- WB: MCF7 and HEK-293 cell lysates; Mouse skeletal muscle lysate. IHC-P: Human adrenal gland tissue and ovarian cancer tissues. ICC/IF: MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab223119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/50 - 1/200. | |
| WB | 1/500 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa). | |
| IHC-P | 1/20 - 1/200. |
Target
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Function
Required for mitochondrial translation, possibly by coordinating the assembly or maintenance of the mitochondrial ribosome (PubMed:23022098, PubMed:25604853). -
Involvement in disease
Combined oxidative phosphorylation deficiency 11 -
Sequence similarities
Belongs to the RMD1/sif2 family. -
Cellular localization
Mitochondrion. May be localized in mitochondrial RNA granules (PubMed:25604853). - Information by UniProt
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Database links
- Entrez Gene: 55005 Human
- Entrez Gene: 66084 Mouse
- SwissProt: Q9NWS8 Human
- SwissProt: Q8CI78 Mouse
- Unigene: 486835 Human
- Unigene: 744622 Human
- Unigene: 153111 Mouse
- Unigene: 313137 Mouse
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Alternative names
- bA351K16 antibody
- bA351K16.3 antibody
- C6orf96 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-RMND1 antibody (ab223119)MCF7 (human breast adenocarcinoma cell line) cells stained for RMND1 (green) using ab223119 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RMND1 antibody (ab223119)Paraffin-embedded human ovarian cancer tissue stained for RMND1 using ab223119 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RMND1 antibody (ab223119)Paraffin-embedded human adrenal gland tissue stained for RMND1 using ab223119 at 1/100 dilution in immunohistochemical analysis.
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Western blot - Anti-RMND1 antibody (ab223119)All lanes : Anti-RMND1 antibody (ab223119) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 3 : Mouse skeletal muscle lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 51 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
ab223119 has not yet been referenced specifically in any publications.
