Overview

  • Product name

    Anti-RNA Helicase A antibody
    See all RNA Helicase A primary antibodies
  • Description

    Rabbit polyclonal to RNA Helicase A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Orangutan
  • Immunogen

    Synthetic peptide within Human RNA Helicase A aa 200-250. The exact sequence is proprietary. CAA71668.1
    Database link: Q08211

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysate. IHC-P: Human prostate carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab245439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability.
    • Sequence similarities

      Belongs to the DEAD box helicase family. DEAH subfamily.
      Contains 2 DRBM (double-stranded RNA-binding) domains.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Domain

      The MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1.
    • Post-translational
      modifications

      Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
      May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • ATP dependent RNA helicase A antibody
      • ATP-dependent RNA helicase A antibody
      • DDX 9 antibody
      • DDX9 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 9 antibody
      • DEAD/H box 9 antibody
      • DEAD/H box polypeptide 9 antibody
      • DEAH (Asp Glu Ala His) box polypeptide 9 antibody
      • DEAH box polypeptide 9 antibody
      • DEAH box protein 9 antibody
      • DHX 9 antibody
      • dhx9 antibody
      • DHX9_HUMAN antibody
      • Leukophysin antibody
      • LKP antibody
      • NDH 2 antibody
      • NDH II antibody
      • NDH2 antibody
      • NDHII antibody
      • Nuclear DNA helicase II antibody
      • RHA antibody
      see all

    Images

    • Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for RNA Helicase A with ab245439 at a 1/1000 dilution in immunohistochemical analysis. DAB staining.

    • All lanes : Anti-RNA Helicase A antibody (ab245439) at 0.1 µg/ml

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : HEK-293T (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

      Exposure time: 10 seconds

    References

    ab245439 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab245439.
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