Anti-RNA Helicase A antibody (ab26271)
Key features and details
- Rabbit polyclonal to RNA Helicase A
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-RNA Helicase A antibody
See all RNA Helicase A primary antibodies -
Description
Rabbit polyclonal to RNA Helicase A -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Monkey, African green monkey
-
Immunogen
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab26271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 141,149 kDa (predicted molecular weight: 141 kDa). This antibody detects a band at 141 kDa in human cell lysates and 149 kDa in mouse cell lysates. |
Target
-
Function
Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability. -
Sequence similarities
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Domain
The MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1. -
Post-translational
modificationsMethylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 281115 Cow
- Entrez Gene: 1660 Human
- Entrez Gene: 13211 Mouse
- Omim: 603115 Human
- SwissProt: Q28141 Cow
- SwissProt: Q08211 Human
- SwissProt: O70133 Mouse
- Unigene: 191518 Human
see all -
Alternative names
- ATP dependent RNA helicase A antibody
- ATP-dependent RNA helicase A antibody
- DDX 9 antibody
see all
Images
-
Western blot - Anti-RNA Helicase A antibody (ab26271)All lanes : Anti-RNA Helicase A antibody (ab26271) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 141 kDa
Observed band size: 141,149 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab26271 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
-
Western blot - Anti-RNA Helicase A antibody (ab26271)Anti-RNA Helicase A antibody (ab26271) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 141 kDa
Observed band size: 141 kDa -
Western blot - Anti-RNA Helicase A antibody (ab26271)This image is courtesy of an Abreview submitted by Mr. Miguel Fidalgo.All lanes : Anti-RNA Helicase A antibody (ab26271) at 1/1000 dilution
All lanes : Human embryonic stem cell nuclear lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Donkey anti-Rabbit HRP IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 141 kDa
Exposure time: 30 seconds
Protocols
References (34)
ab26271 has been referenced in 34 publications.
- Bao G et al. Long noncoding RNA CERS6-AS1 functions as a malignancy promoter in breast cancer by binding to IGF2BP3 to enhance the stability of CERS6 mRNA. Cancer Med 9:278-289 (2020). PubMed: 31701672
- Yan D et al. Circular RNA circPICALM sponges miR-1265 to inhibit bladder cancer metastasis and influence FAK phosphorylation. EBioMedicine 48:316-331 (2019). PubMed: 31648990
- Sharma NR et al. KSHV RNA-binding protein ORF57 inhibits P-body formation to promote viral multiplication by interaction with Ago2 and GW182. Nucleic Acids Res 47:9368-9385 (2019). PubMed: 31400113
- Fu K et al. Biological and RNA regulatory function of MOV10 in mammalian germ cells. BMC Biol 17:39 (2019). PubMed: 31088452
- Murat P et al. RNA G-quadruplexes at upstream open reading frames cause DHX36- and DHX9-dependent translation of human mRNAs. Genome Biol 19:229 (2018). PubMed: 30591072
