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Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human RNA Helicase A.
(Peptide available as ab27786.)
Our Abpromise guarantee covers the use of ab26271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2 µg/mg of lysate.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 141,149 kDa (predicted molecular weight: 141 kDa).
This antibody detects a band at 141 kDa in human cell lysates and 149 kDa in mouse cell lysates.
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab26271 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Image courtesy of Human Protein Atlas . ab26271 staining RNA helicase A in human stomach, showing a predominantly nuclear staining pattern in glandular cells. Paraffin embedded human stomach tissue was incubated with ab26271 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab26271 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org
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