Anti-RNA Helicase A antibody (ab26271)
- Datasheet
- References (31)
- Protocols
Overview
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Product name
Anti-RNA Helicase A antibody
See all RNA Helicase A primary antibodies -
Description
Rabbit polyclonal to RNA Helicase A -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human, African green monkey
Predicted to work with: Cow
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Immunogen
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Positive control
- WB HeLa, HEK293 and NIH 3T3 whole cell lysates; Human embryonic stem cell nuclear lysate. ICC/IF: HeLa cells. IHC-P: Human stomach tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab26271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2 µg/mg of lysate. | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 141,149 kDa (predicted molecular weight: 141 kDa). This antibody detects a band at 141 kDa in human cell lysates and 149 kDa in mouse cell lysates. |
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| ICC/IF | 1/1000. |
Target
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Function
Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability. -
Sequence similarities
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Domain
The MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1. -
Post-translational
modificationsMethylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 281115 Cow
- Entrez Gene: 1660 Human
- Entrez Gene: 13211 Mouse
- Omim: 603115 Human
- SwissProt: Q28141 Cow
- SwissProt: Q08211 Human
- SwissProt: O70133 Mouse
- Unigene: 191518 Human
see all -
Alternative names
- ATP dependent RNA helicase A antibody
- ATP-dependent RNA helicase A antibody
- DDX 9 antibody
see all
Images
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Western blot - Anti-RNA Helicase A antibody (ab26271)All lanes : Anti-RNA Helicase A antibody (ab26271) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 141 kDa
Observed band size: 141,149 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab26271 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Western blot - Anti-RNA Helicase A antibody (ab26271)Anti-RNA Helicase A antibody (ab26271) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 141 kDa
Observed band size: 141 kDa -
Western blot - Anti-RNA Helicase A antibody (ab26271)This image is courtesy of an Abreview submitted by Mr. Miguel Fidalgo.All lanes : Anti-RNA Helicase A antibody (ab26271) at 1/1000 dilution
All lanes : Human embryonic stem cell nuclear lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Donkey anti-Rabbit HRP IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 141 kDa
Exposure time: 30 seconds
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Immunocytochemistry/ Immunofluorescence - Anti-RNA Helicase A antibody (ab26271)This image is courtesy of an Abreview submitted by Dr Kirk McManusab26271, at 1/1000 dilution, staining Human RNA Helicase A in HeLa cells by immunofluorescence. Secondary antibody: Goat anti-Rabbit conjugated to Cy3; Counter-Stain: DAPI; -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA Helicase A antibody (ab26271)Image courtesy of Human Protein Atlas . ab26271 staining RNA helicase A in human stomach, showing a predominantly nuclear staining pattern in glandular cells. Paraffin embedded human stomach tissue was incubated with ab26271 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab26271 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org
Protocols
References
This product has been referenced in:
- Fu K et al. Biological and RNA regulatory function of MOV10 in mammalian germ cells. BMC Biol 17:39 (2019). Read more (PubMed: 31088452) »
- Murat P et al. RNA G-quadruplexes at upstream open reading frames cause DHX36- and DHX9-dependent translation of human mRNAs. Genome Biol 19:229 (2018). Read more (PubMed: 30591072) »
