Overview

  • Product name
    Anti-RNA Helicase A antibody
    See all RNA Helicase A primary antibodies
  • Description
    Rabbit polyclonal to RNA Helicase A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human, African green monkey
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human RNA Helicase A.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab27786.)

  • Positive control
    • WB HeLa, HEK293 and NIH 3T3 whole cell lysates; Human embryonic stem cell nuclear lysate. ICC/IF: HeLa cells. IHC-P: Human stomach tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab26271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2 µg/mg of lysate.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 141,149 kDa (predicted molecular weight: 141 kDa).

This antibody detects a band at 141 kDa in human cell lysates and 149 kDa in mouse cell lysates.
ICC/IF 1/1000.

Target

  • Function
    Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DEAH subfamily.
    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Domain
    The MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1.
  • Post-translational
    modifications
    Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
    May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • ATP dependent RNA helicase A antibody
    • ATP-dependent RNA helicase A antibody
    • DDX 9 antibody
    • DDX9 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 9 antibody
    • DEAD/H box 9 antibody
    • DEAD/H box polypeptide 9 antibody
    • DEAH (Asp Glu Ala His) box polypeptide 9 antibody
    • DEAH box polypeptide 9 antibody
    • DEAH box protein 9 antibody
    • DHX 9 antibody
    • dhx9 antibody
    • DHX9_HUMAN antibody
    • Leukophysin antibody
    • LKP antibody
    • NDH 2 antibody
    • NDH II antibody
    • NDH2 antibody
    • NDHII antibody
    • Nuclear DNA helicase II antibody
    • RHA antibody
    see all

Images

  • Western blot - Anti-RNA Helicase A antibody (ab26271)
    Western blot - Anti-RNA Helicase A antibody (ab26271)
    All lanes : Anti-RNA Helicase A antibody (ab26271) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 141 kDa
    Observed band size: 141,149 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes


    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab26271 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Western blot - Anti-RNA Helicase A antibody (ab26271)
    Western blot - Anti-RNA Helicase A antibody (ab26271)
    Anti-RNA Helicase A antibody (ab26271) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 141 kDa
    Observed band size: 141 kDa

  • Western blot - Anti-RNA Helicase A antibody (ab26271)
    Western blot - Anti-RNA Helicase A antibody (ab26271)This image is courtesy of an Abreview submitted by Mr. Miguel Fidalgo.
    All lanes : Anti-RNA Helicase A antibody (ab26271) at 1/1000 dilution

    All lanes : Human embryonic stem cell nuclear lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Donkey anti-Rabbit HRP IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 141 kDa


    Exposure time: 30 seconds

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-RNA Helicase A antibody (ab26271)
    Immunocytochemistry/ Immunofluorescence - Anti-RNA Helicase A antibody (ab26271)This image is courtesy of an Abreview submitted by Dr Kirk McManus
    ab26271, at 1/1000 dilution, staining Human RNA Helicase A in HeLa cells by immunofluorescence. Secondary antibody: Goat anti-Rabbit conjugated to Cy3; Counter-Stain: DAPI;

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA Helicase A antibody (ab26271)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA Helicase A antibody (ab26271)

    Image courtesy of Human Protein Atlas . ab26271 staining RNA helicase A in human stomach, showing a predominantly nuclear staining pattern in glandular cells. Paraffin embedded human stomach tissue was incubated with ab26271 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab26271 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org

References

This product has been referenced in:
  • Sekiba K  et al. DHX9 regulates production of hepatitis B virus-derived circular RNA and viral protein levels. Oncotarget 9:20953-20964 (2018). Read more (PubMed: 29765512) »
  • Chi B  et al. Interactome analyses revealed that the U1 snRNP machinery overlaps extensively with the RNAP II machinery and contains multiple ALS/SMA-causative proteins. Sci Rep 8:8755 (2018). Read more (PubMed: 29884807) »

See all 21 Publications for this product

Publishing research using ab26271? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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11 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-RNA Helicase A antibody

 Excellent
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Human Cell lysate - nuclear (human embryonic stem cells)
Specification
human embryonic stem cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
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Username

Mr. Miguel Fidalgo

Verified customer

Submitted Oct 27 2014

Immunoprecipitation abreview for Anti-RNA Helicase A antibody

 Good
Abreviews
Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Total protein in input
1000 µg
Read More
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Username

Abcam user community

Verified customer

Submitted Oct 29 2013

Western blot abreview for Anti-RNA Helicase A antibody

 Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - other (HeLa)
Specification
HeLa
Treatment
cytosolic and nuclear fractions
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Username

Abcam user community

Verified customer

Submitted Oct 29 2013

I would like to knowmore about this Mouse monoclonal anti-RHA (later designated anti-DM-RHA) antibody.

Is this antibody able to detect unmethylated form RHA in the cytosol, what's the difference from the antibody that detect RHA in the nucle...

Read More

Thank you for contacting us.

The antibody ab26271 should be able to detect RHA in the cytoplasm. It is raised against a sequence of amino acids that is present in RHA when it is in the nucleus and when it is in the cytoplasm. That will be th...

Read More

Hi,
Could you share me the % identities and number of gaps of the BLASTp between the recognized epitope by your Anti-RNA Helicase A, ab26271 against the sequence of the bovine DDX9 (uniprot Q28141), please?

--

Thank you for contacting us. The positives for the immunogen sequence of ab26271 and the sequence of bovine DHX9 bovine are 17/17 (100%) and gaps 0/17 (0%).

I hope this information is helpful to you. Please do not hesitate to contact us if you...

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Western blot abreview for Anti-RNA Helicase A antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (HeLa cytosolic extract)
Loading amount
50 µg
Specification
HeLa cytosolic extract
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
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Username

Abcam user community

Verified customer

Submitted Jan 05 2010

Immunoprecipitation abreview for Anti-RNA Helicase A antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - other (HeLa cytosolic extract)
Total protein in input
500 µg
Specification
HeLa cytosolic extract
Immuno-precipitation step
Protein A
Read More
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Username

Abcam user community

Verified customer

Submitted Nov 02 2009

Immunoprecipitation abreview for Anti-RNA Helicase A antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
African Green Monkey Cell lysate - whole cell (CV-1, COS-7)
Total protein in input
1000 µg
Specification
CV-1, COS-7
Immuno-precipitation step
Protein G
Read More
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Username

Abcam user community

Verified customer

Submitted Dec 19 2008

Western blot abreview for Anti-RNA Helicase A antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF, NIH-3T3)
Loading amount
20 µg
Specification
MEF, NIH-3T3
Gel Running Conditions
Reduced Denaturing (7.5% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
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Username

Abcam user community

Verified customer

Submitted Apr 01 2008

Western blot abreview for Anti-RNA Helicase A antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293T, HeLa)
Loading amount
20 µg
Specification
293T, HeLa
Gel Running Conditions
Reduced Denaturing (7.5% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted Apr 01 2008

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