Anti-RNA Helicase A antibody (ab70777)

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Overview

  • Product name

    Anti-RNA Helicase A antibody
    See all RNA Helicase A primary antibodies

  • Description

    Rabbit polyclonal to RNA Helicase A

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan

  • Immunogen

    Synthetic peptide corresponding to a region between residue 1220 and the C-terminus (residue 1270) of human RNA Helicase A (CAA71668.1)

  • Positive control

    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 141 kDa (predicted molecular weight: 141 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability.

  • Sequence similarities

    Belongs to the DEAD box helicase family. DEAH subfamily.
    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.

  • Domain

    The MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1.

  • Post-translational
    modifications

    Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
    May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • ATP dependent RNA helicase A antibody
      • ATP-dependent RNA helicase A antibody
      • DDX 9 antibody
      • DDX9 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 9 antibody
      • DEAD/H box 9 antibody
      • DEAD/H box polypeptide 9 antibody
      • DEAH (Asp Glu Ala His) box polypeptide 9 antibody
      • DEAH box polypeptide 9 antibody
      • DEAH box protein 9 antibody
      • DHX 9 antibody
      • dhx9 antibody
      • DHX9_HUMAN antibody
      • Leukophysin antibody
      • LKP antibody
      • NDH 2 antibody
      • NDH II antibody
      • NDH2 antibody
      • NDHII antibody
      • Nuclear DNA helicase II antibody
      • RHA antibody
      see all

    Images

    • Western blot - Anti-RNA Helicase A antibody (ab70777)
      Western blot - Anti-RNA Helicase A antibody (ab70777)
      All lanes : Anti-RNA Helicase A antibody (ab70777) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Predicted band size: 141 kDa
      Observed band size: 141 kDa


      Exposure time: 10 seconds
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA Helicase A antibody (ab70777)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA Helicase A antibody (ab70777)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling RNA Helicase A with ab70777 at 1/200 (1µg/ml). Detection: DAB.
    • Immunoprecipitation - Anti-RNA Helicase A antibody (ab70777)
      Immunoprecipitation - Anti-RNA Helicase A antibody (ab70777)
      Detection of RNA Helicase A by Western Blot of Immunprecipitate. ab70777 at 1µg/ml staining RNA Helicase A in HeLa whole cell lysate immunoprecipitated using ab70777 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

    References

    This product has been referenced in:

    • Booy EP  et al. Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. Nucleic Acids Res 46:11575-11591 (2018). Read more (PubMed: 30247708) »
    • Santos S  et al. Virus-producing cells determine the host protein profiles of HIV-1 virion cores. Retrovirology 9:65 (2012). WB . Read more (PubMed: 22889230) »
    See all 2 Publications for this product

    Publishing research using ab70777? Please let us know so that we can cite the reference in this datasheet.

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