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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Other
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Anti-RNA Helicase A antibody (ab70777)

  • Datasheet
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Western blot - Anti-RNA Helicase A antibody (ab70777)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA Helicase A antibody (ab70777)
  • Immunoprecipitation - Anti-RNA Helicase A antibody (ab70777)

Key features and details

  • Rabbit polyclonal to RNA Helicase A
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human RNA Helicase A protein (ab114300)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-RNA Helicase A antibody
    See all RNA Helicase A primary antibodies
  • Description

    Rabbit polyclonal to RNA Helicase A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1220 and the C-terminus (residue 1270) of human RNA Helicase A (CAA71668.1)

  • Positive control

    • HeLa and 293T whole cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab70777 was affinity purified using an epitope specific to RNA Helicase A immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human RNA Helicase A protein (ab114300)

Applications

Our Abpromise guarantee covers the use of ab70777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 141 kDa (predicted molecular weight: 141 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability.
  • Sequence similarities

    Belongs to the DEAD box helicase family. DEAH subfamily.
    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Domain

    The MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1.
  • Post-translational
    modifications

    Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
    May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm.
  • Target information above from: UniProt accession Q08211 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1660 Human
    • Omim: 603115 Human
    • SwissProt: Q08211 Human
    • SwissProt: Q5R874 Orangutan
    • Unigene: 191518 Human
    • Alternative names

      • ATP dependent RNA helicase A antibody
      • ATP-dependent RNA helicase A antibody
      • DDX 9 antibody
      • DDX9 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 9 antibody
      • DEAD/H box 9 antibody
      • DEAD/H box polypeptide 9 antibody
      • DEAH (Asp Glu Ala His) box polypeptide 9 antibody
      • DEAH box polypeptide 9 antibody
      • DEAH box protein 9 antibody
      • DHX 9 antibody
      • dhx9 antibody
      • DHX9_HUMAN antibody
      • Leukophysin antibody
      • LKP antibody
      • NDH 2 antibody
      • NDH II antibody
      • NDH2 antibody
      • NDHII antibody
      • Nuclear DNA helicase II antibody
      • RHA antibody
      see all

    Images

    • Western blot - Anti-RNA Helicase A antibody (ab70777)
      Western blot - Anti-RNA Helicase A antibody (ab70777)
      All lanes : Anti-RNA Helicase A antibody (ab70777) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Predicted band size: 141 kDa
      Observed band size: 141 kDa


      Exposure time: 10 seconds
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA Helicase A antibody (ab70777)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA Helicase A antibody (ab70777)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling RNA Helicase A with ab70777 at 1/200 (1µg/ml). Detection: DAB.
    • Immunoprecipitation - Anti-RNA Helicase A antibody (ab70777)
      Immunoprecipitation - Anti-RNA Helicase A antibody (ab70777)
      Detection of RNA Helicase A by Western Blot of Immunprecipitate. ab70777 at 1µg/ml staining RNA Helicase A in HeLa whole cell lysate immunoprecipitated using ab70777 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (2)

    Publishing research using ab70777? Please let us know so that we can cite the reference in this datasheet.

    ab70777 has been referenced in 2 publications.

    • Booy EP  et al. Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. Nucleic Acids Res 46:11575-11591 (2018). PubMed: 30247708
    • Santos S  et al. Virus-producing cells determine the host protein profiles of HIV-1 virion cores. Retrovirology 9:65 (2012). WB . PubMed: 22889230

    Customer reviews and Q&As

    Show All Reviews Q&A
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    There are currently no Customer reviews or Questions for ab70777.
    Please use the links above to contact us or submit feedback about this product.

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