The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/10000 - 1/50000. Detects a band of approximately 141 kDa (predicted molecular weight: 141 kDa).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability.
Belongs to the DEAD box helicase family. DEAH subfamily. Contains 2 DRBM (double-stranded RNA-binding) domains. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
The MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1.
Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization. May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm.
Immunohistochemical analysis of paraffin embedded Mouse colon tissue labeling RNA Helicase A with ab183731 at a 1/100 dilution. Prediluted HRP conjugated Rabbit IgG secondary used. Counterstained with Hematoxylin.
Flow Cytometry - Anti-RNA Helicase A antibody [EPR13521] (ab183731)
Flow cytometry analysis of HeLa cells using ab183731 at a 1/10 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.
Immunocytochemistry - Anti-RNA Helicase A antibody [EPR13521] (ab183731)
Immunocytochemistry analysis of HeLa cells (fixative -20℃ Acetone) labeling RNA Helicase A with ab183731 at a 1/250 dilution.Goat anti rabbit IgG (Alexa Fluor® 488) secondary used at a 1/200 diution.
Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labeling RNA Helicase A with ab183731 at a 1/100 dilution. Prediluted HRP conjugated Rabbit IgG secondary used. Counterstained with Hematoxylin.
Western blot - Anti-RNA Helicase A antibody [EPR13521] (ab183731)
All lanes : Anti-RNA Helicase A antibody [EPR13521] (ab183731) at 1/50000 dilution
Lane 1 : HeLa lysate Lane 2 : Jurkat lysate Lane 3 : 293 lysate Lane 4 : BxPC-3 lysate
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution