Product nameAnti-RNA polymerase II antibody
See all RNA polymerase II primary antibodies
DescriptionRabbit polyclonal to RNA polymerase II
Tested applicationsSuitable for: IP, ChIPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human RNA polymerase II aa 754-804. The exact sequence is proprietary. NP_000928.1
Database link: P24928
- IP: HEK-293T whole cell lysate. ChIP: Chromatin prepared from HeLa cells.
Anti-RNA polymerase II antibody (ab264350) has not performed satisfactorily when used for WB of RNA polymerase II in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of RNA polymerase II.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264350 in the following tested applications.
|IP||Use at 2-10 µg/mg of lysate.|
|ChIP||Use at an assay dependent concentration.
8 μg / 15cm2 plate of HeLa cells.
RelevanceDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. RNA polymerase II has two physiologically important phosphorylation sites; ser 2 and ser 5. Both sites are found in the heptapeptide repeat YSPTSPS at the C terminal domain.
- DNA directed RNA polymerase II A antibody
- DNA directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit antibody
- DNA directed RNA polymerase II polypeptide A antibody
Chromatin isolated from one 15 cm plate of HeLa (human epithelial cell line from cervix adenocarcinoma) cells was immunoprecipitated with 8.0 µg of anti-RNA polymerase II antibodies and PCR amplified.
RNA polymerase II was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) using ab264350 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using an alternative RNA polymerase II antibody.
Lane 1: ab264350 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes.
ab264350 has not yet been referenced specifically in any publications.