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Immunocytochemistry/ Immunofluorescence abreview for Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [EPR18855]

Excellent
Abreviews
Go to datasheet (ab193468)
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Zebrafish Cell (Dissociated gastrula embryo cells)
Permeabilization
Yes - 0.5% Triton x-100, 10 minutes
Specification
Dissociated gastrula embryo cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA, 0.1% Tween-20 in D-PBS
Dilution
1/1000

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: ThermoFisher A-11037
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

Additional data

Additional Notes
Gave speckled signal comparable to "classic" H5 antibody (ab24758, discontinued), but more consistent staining.

Dr. Lennart Hilbert

Verified customer

Submitted Dec 20 2016