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Thank you again for your infor about the Ab9050 antibody. I have just placed an order of 6 vials along with orders of several antibodies (total ˜18 vials) today, and hopefully you will receive the order soon.
Here I have a question about three other antibodies we purchased on/around April 5. We ordered three RNA polymerase antibodies ( ab5408, ab5131, ab5095), all of which are ChIP-grade antibodies. We tested the antibodies along with Ab9050 H3K36me3 antibody for ChIP at the same time, although we got very strong signals with Ab9050 (2-3% of Input), all the Pol II antibodies showed only background binding similar to IgG control(0.0004-0.001% of Input). We’ve used the ab5131 and ab5095 antibodies in the past (1.5-2 years ago, the same catalog #) and it gave us reasonable signals (0.3-1% of Input). I believe the current ones are from different lots and I would like to know if you have received similar concerns about these Pol II antibodies recently. If you know of Pol II antibodies from other catalog or lots are working properly for ChIP, I will very much appreciate if you could provide me with that information, or send us a replacement for these three antibodies.
I have a related question: we would like to store antibodies that we ordered several tubes in –80. Shall we snap freeze them with liquid nitrogen before putting them into –80 freezer?
Asked on Apr 18 2012
Thank you for your emails and I apologize for the delay getting back to you.
I am sorry to hear about the trouble with these RNA polymerase antibodies as well. I've looked through our records and we have not received similar concerns, but I'll be happy to get in touch with our ChIP expert in the lab and ask if any other lots in stock perform better in ChIP. I will forward any information I obtain from him on to you, and you can decide which lots you would like as replacements.
Could you tell me what dilution or concentration you're using for each antibody in ChIP? What kind of cells are you studying, and is the chromatin fresh? What genes are used as the positive controls? This information will assist us in our quality investigation of the lots you're currently using.
Regarding storage at -80C, you do not need to snap freeze in liquid nitrogen prior to putting them in the freezer. Please be sure to aliquot them, however.
I hope this information will be helpful, but I will be back in touch soon with more information about the current lots of these antibodies that we have in stock.
Answered on Apr 18 2012