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    rna-polymerase-ii-ctd-repeat-ysptsps-phospho-s5-antibody-ab5131.pdf

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Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol II Transcription
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Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131)

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  • SDS
Reviews (48)Q&A (7)References (390)

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ChIP - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131)
  • ChIP - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131)
  • Immunocytochemistry - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131)
  • Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131)
  • Immunocytochemistry/ Immunofluorescence - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131)
  • Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131)
  • Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131)

Key features and details

  • Rabbit polyclonal to RNA polymerase II CTD repeat YSPTSPS (phospho S5)
  • Suitable for: IHC-Fr, IHC-P, ELISA, IP, WB, ChIP, ICC/IF, IHC - Wholemount
  • Reacts with: Mouse, Rat, Human, Saccharomyces cerevisiae, Xenopus laevis, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe, Zebrafish
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [EPR19015] (ab193467)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody
    See all RNA polymerase II CTD repeat YSPTSPS primary antibodies
  • Description

    Rabbit polyclonal to RNA polymerase II CTD repeat YSPTSPS (phospho S5)
  • Host species

    Rabbit
  • Specificity

    This antibody recognises the phosphorylated serine found in the amino acid 5 position of the C-terminal domain repeat YSPTSPS of RNA polymerase II.
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, ELISA, IP, WB, ChIP, ICC/IF, IHC - Wholemountmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Saccharomyces cerevisiae, Xenopus laevis, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe, Zebrafish
    Predicted to work with: a wide range of other species
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab18488)

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab18488)

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab18488)

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab18488)

  • Positive control

    • ICC/IF: HeLa cells. WB: Xenopus laevis whole tissue lysate
  • General notes

    Phosphorylation of RNA polymerase II's largest subunit C-terminal domain (CTD) is a key event during mRNA metabolism.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    Phosphorylation of RNA polymerase II's largest subunit C-terminal domain (CTD) is a key event during mRNA metabolism.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Polymerase
    • Epigenetics and Nuclear Signaling
    • Transcription
    • RNA polymerase

Associated products

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Control Peptide

    • S. cerevisiae RNA polymerase II CTD repeat YSPTSPS (phospho S2) peptide (ab12793)
  • Immunizing Peptide (Blocking)

    • Human RNA polymerase II CTD repeat YSPTSPS (phospho S5) peptide (ab18488)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human RNA polymerase II CTD repeat YSPTSPS protein (ab81834)
    • Recombinant human RNA polymerase II CTD repeat YSPTSPS protein (ab81888)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ELISA (1)
Use at an assay dependent concentration.
IP (1)
Use at an assay dependent concentration.
WB (15)
1/1000. Detects a band of approximately 240 kDa (predicted molecular weight: 217 kDa).Can be blocked with Human RNA polymerase II CTD repeat YSPTSPS (phospho S5) peptide (ab18488).
ChIP (17)
Use 2 µg for 25 µg of chromatin.
ICC/IF (9)
Use a concentration of 1 µg/ml.
IHC - Wholemount (2)
Use at an assay dependent concentration.
Notes
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
1/1000. Detects a band of approximately 240 kDa (predicted molecular weight: 217 kDa).Can be blocked with Human RNA polymerase II CTD repeat YSPTSPS (phospho S5) peptide (ab18488).
ChIP
Use 2 µg for 25 µg of chromatin.
ICC/IF
Use a concentration of 1 µg/ml.
IHC - Wholemount
Use at an assay dependent concentration.

Target

  • Function

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.
  • Sequence similarities

    Belongs to the RNA polymerase beta' chain family.
  • Domain

    The C-terminal domain (CTD) serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.
  • Post-translational
    modifications

    The tandem heptapeptide repeats in the C-terminal domain (CTD) can be highly phosphorylated. The phosphorylation activates Pol II. Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the heptapeptide repeat and is mediated, at least, by CDK7 and CDK9. CDK7 phosphorylation of POLR2A associated with DNA promotes transcription initiation by triggering dissociation from DNA. Phosphorylation also takes place at 'Ser-7' of the heptapeptide repeat, which is required for efficient transcription of snRNA genes and processing of the transcripts. The phosphorylation state is believed to result from the balanced action of site-specific CTD kinases and phosphatases, and a 'CTD code' that specifies the position of Pol II within the transcription cycle has been proposed. Dephosphorylated by the protein phosphatase CTDSP1.
    Among tandem heptapeptide repeats of the C-terminal domain (CTD) some do not match the Y-S-P-T-S-P-S consensus, the seventh serine residue 'Ser-7' being replaced by a lysine. 'Lys-7' in these non-consensus heptapeptide repeats can be alternatively acetylated, methylated and dimethylated. EP300 is one of the enzyme able to acetylate 'Lys-7'. Acetylation at 'Lys-7' of non-consensus heptapeptide repeats is associated with 'Ser-2' phosphorylation and active transcription. It may regulate initiation or early elongation steps of transcription specially for inducible genes.
    Methylated at Arg-1810 prior to transcription initiation when the CTD is hypophosphorylated, phosphorylation at Ser-1805 and Ser-1808 preventing this methylation. Symmetrically or asymmetrically dimethylated at Arg-1810 by PRMT5 and CARM1 respectively. Symmetric or asymmetric dimethylation modulates interactions with CTD-binding proteins like SMN1/SMN2 and TDRD3. SMN1/SMN2 interacts preferentially with the symmetrically dimethylated form while TDRD3 interacts with the asymmetric form. Through the recruitment of SMN1/SMN2, symmetric dimethylation is required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination. CTD dimethylation may also facilitate the expression of select RNAs. Among tandem heptapeptide repeats of the C-terminal domain (CTD) some do not match the Y-S-P-T-S-P-S consensus, the seventh serine residue 'Ser-7' being replaced by a lysine. 'Lys-7' in these non-consensus heptapeptide repeats can be alternatively acetylated, methylated and dimethylated. Methylation occurs in the earliest transcription stages and precedes or is concomitant to 'Ser-5' and 'Ser-7' phosphorylation.
    Ubiquitinated by WWP2 leading to proteasomal degradation (By similarity). Following UV treatment, the elongating form of RNA polymerase II (RNA pol IIo) is ubiquitinated UV damage sites without leading to degradation: ubiquitination is facilitated by KIAA1530/UVSSA and promotes RNA pol IIo backtracking to allow access to the nucleotide excision repair machinery.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P24928 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 177190 Caenorhabditis elegans
    • Entrez Gene: 32100 Drosophila melanogaster
    • Entrez Gene: 5430 Human
    • Entrez Gene: 20020 Mouse
    • Entrez Gene: 363633 Rat
    • Entrez Gene: 851415 Saccharomyces cerevisiae
    • Omim: 180660 Human
    • SwissProt: P18616 Arabidopsis thaliana
    • SwissProt: P16356 Caenorhabditis elegans
    • SwissProt: P04052 Drosophila melanogaster
    • SwissProt: P24928 Human
    • SwissProt: P08775 Mouse
    • SwissProt: P04050 Saccharomyces cerevisiae
    • Unigene: 2925 Drosophila melanogaster
    • Unigene: 270017 Human
    • Unigene: 16533 Mouse
    see all
  • Alternative names

    • DNA directed RNA polymerase II A antibody
    • DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit antibody
    • DNA-directed RNA polymerase II subunit A antibody
    • DNA-directed RNA polymerase II subunit RPB1 antibody
    • DNA-directed RNA polymerase III largest subunit antibody
    • hRPB220 antibody
    • hsRPB1 antibody
    • POLR2 antibody
    • Polr2a antibody
    • POLRA antibody
    • Polymerase (RNA) II (DNA directed) polypeptide A 220kDa antibody
    • Polymerase (RNA) II (DNA directed) polypeptide A antibody
    • RNA polymerase II subunit B1 antibody
    • RNA-directed RNA polymerase II subunit RPB1 antibody
    • RPB1 antibody
    • RPB1_HUMAN antibody
    • RPBh1 antibody
    • RpIILS antibody
    • RPO2 antibody
    • RPOL2 antibody
    see all

Images

  • ChIP - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131)
    ChIP - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131)Murgatroyd et al PLoS One. 2014 Mar 5;9(3):e90277. doi: 10.1371/journal.pone.0090277. eCollection 2014. Fig S2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Chromatin from cultured cells, mouse PVN punches (individual pools formed from groups of 3) or whole hypothalami dissected from fresh brains were cross linked, disrupted by sonification and purified.

    The binding of Suz12 to wingless (Wnt1), a beta-catenin dependent developmental regulator and to the RNA polymerase II promoter (RNAPII), a housekeeping gene, served as positive and negative controls, respectively, in these experiments.

  • ChIP - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131)
    ChIP - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131)

    Chromatin was prepared from U-2 OS (Human bone osteosarcoma epithelial cell line) cells according to the Abcam X-ChIP protocol.

    Cells were fixed with formaldehyde for 10 minutes. The  ChIP was performed with 25 µg of chromatin, 2 µg of  ab5131 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the inactive AFM and F8 promoters, the GAPDH promoter (active) and over the y-Actin gene (active).

    Schematic diagram of the y-Actin gene is shown on the top of the figure. Black boxes represent exons and thin lines represent introns. PCR products are depicted as bars under the gene.

  • Immunocytochemistry - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131)
    Immunocytochemistry - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131)

    ab5131 staining RNA polymerase II CTD repeat YSPTSPS (phospho S5) in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab5131 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 4% paraformaldehyde (10 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131)
    Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131)This image is courtesy of an anonymous Abreview
    All lanes : Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) at 1/5000 dilution

    Lane 1 : Xenopus laevis whole tissue lysate treated with DMSO for 24 hours
    Lane 2 : Xenopus laevis whole tissue lysate treated with CDK inhibitor for 24 hours

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 217 kDa
    Observed band size: 240 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131)
    Immunocytochemistry/ Immunofluorescence - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131)

    ICC/IF image of ab5131 stained HeLa(Human epithelial cell line from cervix adenocarcinoma) cells.

    The cells were fixed in methanol for 5 minutes, permabilized in TBS-T  for 20 minutes and incubated with the antibody (ab5131, 1 µg/ml) for 1 hour at room temperature. 1% BSA / 10% normal goat serum / 0.3M glycine was used to quench auto-fluorescence and block non-specific protein-protein interactions.

    The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131)
    Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131)
    All lanes : Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : S.cerevisiae (Y190) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate with S. cerevisiae RNA polymerase II CTD repeat YSPTSPS peptide (ab12795) at 1 µg/ml
    Lane 4 : S.cerevisiae (Y190) Whole Cell Lysate with S. cerevisiae RNA polymerase II CTD repeat YSPTSPS peptide (ab12795) at 1 µg/ml
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate with Human RNA polymerase II CTD repeat YSPTSPS (phospho S5) peptide (ab18488) at 1 µg/ml
    Lane 6 : S.cerevisiae (Y190) Whole Cell Lysate with Human RNA polymerase II CTD repeat YSPTSPS (phospho S5) peptide (ab18488) at 1 µg/ml
    Lane 7 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate with S. cerevisiae RNA polymerase II CTD repeat YSPTSPS (phospho S2) peptide (ab12793) at 1 µg/ml
    Lane 8 : S.cerevisiae (Y190) Whole Cell Lysate with S. cerevisiae RNA polymerase II CTD repeat YSPTSPS (phospho S2) peptide (ab12793) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 217 kDa


    Exposure time: 30 seconds
  • Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131)
    Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131)
    Lanes 1 & 3-4 : Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) at 1/500 dilution
    Lane 2 : Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) at 1/2000 dilution

    Lanes 1-2 : HeLa nuclear extract
    Lane 3 : HeLa nuclear extract with Human RNA polymerase II CTD repeat YSPTSPS (phospho S5) peptide (ab18488) at 1 µg/ml
    Lane 4 : HeLa nuclear extract with S. cerevisiae RNA polymerase II CTD repeat YSPTSPS peptide (ab12795) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 217 kDa
    Observed band size: 250 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds

Protocols

  • Abreview protocol for ChIP using ab5131

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (390)

Publishing research using ab5131? Please let us know so that we can cite the reference in this datasheet.

ab5131 has been referenced in 390 publications.

  • Li X  et al. Cohesin promotes HSV-1 lytic transcription by facilitating the binding of RNA Pol II on viral genes. Virol J 18:26 (2021). PubMed: 33485391
  • Laffleur B  et al. Noncoding RNA processing by DIS3 regulates chromosomal architecture and somatic hypermutation in B cells. Nat Genet 53:230-242 (2021). PubMed: 33526923
  • Legartová S  et al. The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction. Cells 10:N/A (2021). PubMed: 33535591
  • Bajusz C  et al. The nuclear activity of the actin-binding Moesin protein is necessary for gene expression in Drosophila. FEBS J N/A:N/A (2021). PubMed: 33606336
  • Jang Y  et al. MED1 is a lipogenesis coactivator required for postnatal adipose expansion. Genes Dev 35:713-728 (2021). PubMed: 33888555
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

IHC - Wholemount abreview for Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Caenorhabditis elegans Embryo (C. elegans larvae)
Specification
C. elegans larvae
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 30 2013

IHC - Wholemount abreview for Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Fruit fly (Drosophila melanogaster) Tissue (giant polytene chromosome in salivary glands)
Specification
giant polytene chromosome in salivary glands
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 30 2013

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