Overview

  • Product name

    Anti-RNA polymerase II RPB1 antibody
    See all RNA polymerase II RPB1 primary antibodies
  • Description

    Goat polyclonal to RNA polymerase II RPB1
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Plants, Zebrafish, Rhesus monkey, Gorilla, Soybean, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region within the C terminal repeat region of Human RNA polymerase II RPB1 (NP_000928.1).

  • Positive control

    • 293T, HeLa, Jurkat and NIH3T3 whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab140509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 217 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance

    RPB1 is a subunit of RNA polymerase II which catalyses transcription of RNA from DNA. RPB1 forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • DNA directed RNA polymerase II subunit RPB1 antibody
    • hRPB220 antibody
    • hsRPB1 antibody
    • POLR2 antibody
    • POLR2A antibody
    • POLRA antibody
    • polymerase (RNA) II (DNA directed) polypeptide A, 220kDa antibody
    • RNA polymerase II subunit 1 antibody
    • RNA polymerase II subunit B1 antibody
    • RP02 antibody
    • RPB220 antibody
    • RPBh1 antibody
    • RpIILS antibody
    • RPO21 antibody
    • RPOL2 antibody
    • SUA8 antibody
    see all

Images

  • All lanes : Anti-RNA polymerase II RPB1 antibody (ab140509) at 0.4 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 217 kDa


    Exposure time: 30 seconds
  • All lanes : Anti-RNA polymerase II RPB1 antibody (ab140509) at 1 µg/ml

    Lane 1 : NIH3T3 whole cell lysate at 50 µg
    Lane 2 : NIH3T3 whole cell lysate at 15 µg

    Developed using the ECL technique.

    Predicted band size: 217 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation of RNA polymerase II RPB1 from 293T whole cell lysate usinf ab140509 at 6 µg/mg lysate (1 mg of lysate for IP; 20% of IP loaded). For blotting immunoprecipitated RNA polymerase II RPB1, ab140509 was used at 1 µg/ml.
    Detection: chemiluminescence with an exposure time of 30 seconds.

References

This product has been referenced in:

See all 2 Publications for this product

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