Anti-RNA polymerase II RPB4 antibody (ab186868)
Key features and details
- Rabbit polyclonal to RNA polymerase II RPB4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RNA polymerase II RPB4 antibody
See all RNA polymerase II RPB4 primary antibodies -
Description
Rabbit polyclonal to RNA polymerase II RPB4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human RNA polymerase II RPB4 aa 1-142.
Database link: O15514 -
Positive control
- MCF-7, SW480, SW620, HepG2 and SK-HEPC cell lysates; Mouse spleen lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 16 kDa.
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IHC-P |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 16 kDa. |
IHC-P
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. -
Sequence similarities
Belongs to the eukaryotic RPB4 RNA polymerase subunit family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5433 Human
- Entrez Gene: 69241 Mouse
- Omim: 606017 Human
- SwissProt: O15514 Human
- SwissProt: Q9D7M8 Mouse
- Unigene: 728890 Human
- Unigene: 279898 Mouse
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Alternative names
- DNA directed RNA polymerase II 16 kDa polypeptide antibody
- DNA directed RNA polymerase II subunit D antibody
- DNA directed RNA polymerase II subunit RPB4 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophageal cancer tissue labelling RNA polymerase II RPB4 with ab186868 at 1/100. Magnification: 400x.
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All lanes : Anti-RNA polymerase II RPB4 antibody (ab186868) at 1/500 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : SW480 cell lysate
Lane 3 : SW620 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : SK-HEPC cell lysate
Lane 6 : Mouse spleen tissue lysate
Predicted band size: 16 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab186868 has been referenced in 1 publication.
- Liu B et al. Yeats4 drives ILC lineage commitment via activation of Lmo4 transcription. J Exp Med 216:2653-2668 (2019). PubMed: 31434684