Key features and details
- Rabbit polyclonal to RNA polymerase II RPB4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-RNA polymerase II RPB4 antibody
See all RNA polymerase II RPB4 primary antibodies
DescriptionRabbit polyclonal to RNA polymerase II RPB4
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human RNA polymerase II RPB4 aa 1-142.
Database link: O15514
- MCF-7, SW480, SW620, HepG2 and SK-HEPC cell lysates; Mouse spleen lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab186868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 16 kDa.|
|IHC-P||1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA.
Sequence similaritiesBelongs to the eukaryotic RPB4 RNA polymerase subunit family.
- Information by UniProt
- DNA directed RNA polymerase II 16 kDa polypeptide antibody
- DNA directed RNA polymerase II subunit D antibody
- DNA directed RNA polymerase II subunit RPB4 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophageal cancer tissue labelling RNA polymerase II RPB4 with ab186868 at 1/100. Magnification: 400x.
All lanes : Anti-RNA polymerase II RPB4 antibody (ab186868) at 1/500 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : SW480 cell lysate
Lane 3 : SW620 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : SK-HEPC cell lysate
Lane 6 : Mouse spleen tissue lysate
Predicted band size: 16 kDa
ab186868 has not yet been referenced specifically in any publications.