Overview

  • Product name

    Anti-RNA polymerase II RPB4 antibody
    See all RNA polymerase II RPB4 primary antibodies
  • Description

    Rabbit polyclonal to RNA polymerase II RPB4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human RNA polymerase II RPB4 (internal sequence). The exact sequence is proprietary.
    Database link: O15514

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab229488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 16 kDa.

Target

  • Function

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA.
  • Sequence similarities

    Belongs to the eukaryotic RPB4 RNA polymerase subunit family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA directed RNA polymerase II 16 kDa polypeptide antibody
    • DNA directed RNA polymerase II subunit D antibody
    • DNA directed RNA polymerase II subunit RPB4 antibody
    • DNA-directed RNA polymerase II 16 kDa polypeptide antibody
    • DNA-directed RNA polymerase II subunit D antibody
    • DNA-directed RNA polymerase II subunit rpb4 antibody
    • HSRBP4 antibody
    • HSRPB4 antibody
    • polr2d antibody
    • polymerase RNA II DNA directed polypeptide D antibody
    • RBP4 antibody
    • RNA polymerase II 16 kDa subunit antibody
    • RNA polymerase II subunit B4 antibody
    • RNA polymerase II subunit D antibody
    • RNA polymerase II subunit hsRBP4 antibody
    • RPB16 antibody
    • RPB4_HUMAN antibody
    see all

Images

  • All lanes : Anti-RNA polymerase II RPB4 antibody (ab229488) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 16 kDa



    15% SDS-PAGE

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RNA polymerase II RPB4 (green) using ab229488 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde/PBS for 15 minutes.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229488 has not yet been referenced specifically in any publications.

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