Product nameAnti-RNA polymerase II RPB4 antibody
See all RNA polymerase II RPB4 primary antibodies
DescriptionRabbit polyclonal to RNA polymerase II RPB4
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey
Recombinant fragment within Human RNA polymerase II RPB4 (internal sequence). The exact sequence is proprietary.
Database link: O15514
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. ICC/IF: HeLa cells.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229488 in the following tested applications.
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 16 kDa.|
FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA.
Sequence similaritiesBelongs to the eukaryotic RPB4 RNA polymerase subunit family.
- Information by UniProt
- DNA directed RNA polymerase II 16 kDa polypeptide antibody
- DNA directed RNA polymerase II subunit D antibody
- DNA directed RNA polymerase II subunit RPB4 antibody
All lanes : Anti-RNA polymerase II RPB4 antibody (ab229488) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 16 kDa
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RNA polymerase II RPB4 (green) using ab229488 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde/PBS for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
ab229488 has not yet been referenced specifically in any publications.