Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: 50% Glycerol
Our Abpromise guarantee covers the use of ab51462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 46 kDa.|
FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA.
Sequence similaritiesBelongs to the eukaryotic RPB4 RNA polymerase subunit family.
- Information by UniProt
- SwissProt: Q9VEA5 Drosophila melanogaster
- DNA directed RNA polymerase II 16 kDa polypeptide antibody
- DNA directed RNA polymerase II subunit D antibody
- DNA directed RNA polymerase II subunit RPB4 antibody
ab51462 has not yet been referenced specifically in any publications.