Anti-RNase 7 antibody [3C2] (ab189222)
Key features and details
- Mouse monoclonal [3C2] to RNase 7
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-RNase 7 antibody [3C2]
See all RNase 7 primary antibodies -
Description
Mouse monoclonal [3C2] to RNase 7 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human RNase 7 aa 29-156. (Purified from cell culture supernatant).
Database link: Q9H1E1 -
Positive control
- WB: Human RNase 7 expressed by CHOEBNALT85 cell line. IHC-P: Human skin tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3C2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
1/200 - 1/2000. Predicted molecular weight: 17 kDa.
1/500 to 1/2000 under non-reducing conditions; 1/200 to 1/1000 under reducing conditions. |
Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
1/200 - 1/2000. Predicted molecular weight: 17 kDa. 1/500 to 1/2000 under non-reducing conditions; 1/200 to 1/1000 under reducing conditions. |
Target
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Function
Exhibits a potent RNase activity. Has broad-spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium. -
Tissue specificity
Expressed in various epithelial tissues including skin, respiratory tract, genito-urinary tract and, at a low level, in the gut. Expressed in liver, kidney, skeletal muscle and heart. -
Sequence similarities
Belongs to the pancreatic ribonuclease family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 84659 Human
- Omim: 612484 Human
- SwissProt: Q9H1E1 Human
- Unigene: 525206 Human
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Alternative names
- EC 3.1.27.- antibody
- MGC133220 antibody
- Ribonuclease 7 antibody
see all
Images
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Lane 1 : Anti-RNase 7 antibody [3C2] (ab189222) at 1/500 dilution
Lane 2 : Anti-RNase 7 antibody [3C2] (ab189222) at 1/200 dilution
All lanes : Human RNase 7 expressed by CHOEBNALT85 cell line (cell culture supernatant)
Lysates/proteins at 10 µl per lane.
Predicted band size: 17 kDaLane 1: Non-reduced conditions; Lane 2: Reduced conditions
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Immunohistochemical analysis of paraffin-embedded human skin tissue labeling RNase 7 antibody with ab189222. Biotinylated anti-mouse antibody was used as secondary antibody. Streptavidin-HRP conjugate was used for visualization
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab189222 has not yet been referenced specifically in any publications.