Anti-RNase 7 antibody [4C4] (ab205565)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNase 7 antibody [4C4] (ab205565)](https://www.abcam.com/ps/products/205/ab205565/Images/ab205565-248612-anti-rnase-7-antibody-4c4-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNase 7 antibody [4C4] (ab205565)")
Key features and details
- Mouse monoclonal [4C4] to RNase 7
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-RNase 7 antibody [4C4]
See all RNase 7 primary antibodies -
Description
Mouse monoclonal [4C4] to RNase 7 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human RNase 7. Recombinant Human RNase 7 protein produced by CHO-based proprietary suspension cell line.
Database link: Q9H1E1 -
Positive control
- CHOEBNALT85 cell line lysate. U2OS cells expressing RNase 7. Human skin tissue.
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General notes
Protein previously labeled as Ribonuclease 7.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4C4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab205565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 - 20 µg/ml. | |
| WB | Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 17 kDa. WB in non-reduced conditions. |
|
| ICC/IF | Use a concentration of 0.5 - 20 µg/ml. |
Target
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Function
Exhibits a potent RNase activity. Has broad-spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium. -
Tissue specificity
Expressed in various epithelial tissues including skin, respiratory tract, genito-urinary tract and, at a low level, in the gut. Expressed in liver, kidney, skeletal muscle and heart. -
Sequence similarities
Belongs to the pancreatic ribonuclease family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 84659 Human
- Omim: 612484 Human
- SwissProt: Q9H1E1 Human
- Unigene: 525206 Human
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Alternative names
- EC 3.1.27.- antibody
- MGC133220 antibody
- Ribonuclease 7 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNase 7 antibody [4C4] (ab205565)
Immunohistochemical analysis of paraffin-embedded human skin tissue tissue labeling RNAase 7 with ab205565 at 1/500 dilution. Biotinylated anti-mouse antibody was used as secondary antibody. Streptavidin-HRP conjugate was used for visualization.
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![Western blot - Anti-RNase 7 antibody [4C4] (ab205565)](https://www.abcam.com/ps/products/205/ab205565/Images/ab205565-248610-anti-rnase-7-antibody-4c4-western-blot.jpg)
Western blot - Anti-RNase 7 antibody [4C4] (ab205565)All lanes : Anti-RNase 7 antibody [4C4] (ab205565) at 2 µg/ml
Lane 1 : CHOEBNALT85 cell line lysate; non-reduced conditions at 10 µl
Lane 2 : CHOEBNALT85 cell line lysate; reduced conditions at 10 µl
Lane 3 : Protein ladder
Predicted band size: 17 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-RNase 7 antibody [4C4] (ab205565)](https://www.abcam.com/ps/products/205/ab205565/Images/ab205565-248611-anti-rnase-7-antibody-4c4-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RNase 7 antibody [4C4] (ab205565)Immunofluorescence analysis of U2OS cells labeling RNAase 7 with ab205565 at 0.5 µg/mL. Nuclei were counterstained by DAPI. Left image; U2OS cells expressing RNAase 7. Right image; Negative control (non-transfected U2OS cells).
Protocols
References (1)
ab205565 has been referenced in 1 publication.
- Gazi U et al. Tonsillar antimicrobial peptide (AMP) expression profiles of periodic fever, aphthous stomatitis, pharyngitis, cervical adenitis (PFAPA) patients. Int J Pediatr Otorhinolaryngol 110:100-104 (2018). IHC-P ; Human . PubMed: 29859567
