Anti-RNase 7 antibody [4C4] (ab205565)

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Overview

  • Product name

    Anti-RNase 7 antibody [4C4]
    See all RNase 7 primary antibodies

  • Description

    Mouse monoclonal [4C4] to RNase 7

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human RNase 7. Recombinant Human RNase 7 protein produced by CHO-based proprietary suspension cell line.
    Database link: Q9H1E1

  • Positive control

    • CHOEBNALT85 cell line lysate. U2OS cells expressing RNase 7. Human skin tissue.

  • General notes

    Protein previously labeled as Ribonuclease 7.

Properties

Applications

Our Abpromise guarantee covers the use of ab205565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 20 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 17 kDa.

WB in non-reduced conditions.
ICC/IF Use a concentration of 0.5 - 20 µg/ml.

Target

  • Function

    Exhibits a potent RNase activity. Has broad-spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium.

  • Tissue specificity

    Expressed in various epithelial tissues including skin, respiratory tract, genito-urinary tract and, at a low level, in the gut. Expressed in liver, kidney, skeletal muscle and heart.

  • Sequence similarities

    Belongs to the pancreatic ribonuclease family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • EC 3.1.27.- antibody
      • MGC133220 antibody
      • Ribonuclease 7 antibody
      • ribonuclease, RNase A family, 7 antibody
      • RNAS7_HUMAN antibody
      • RNase 7 antibody
      • RNASE7 antibody
      • SAP 2 antibody
      • SAP-2 antibody
      • Skin derived antimicrobial protein 2 antibody
      • Skin-derived antimicrobial protein 2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNase 7 antibody [4C4] (ab205565)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNase 7 antibody [4C4] (ab205565)

      Immunohistochemical analysis of paraffin-embedded human skin tissue tissue labeling RNAase 7  with ab205565 at 1/500 dilution. Biotinylated anti-mouse antibody was used as secondary antibody. Streptavidin-HRP conjugate was used for visualization.

    • Western blot - Anti-RNase 7 antibody [4C4] (ab205565)
      Western blot - Anti-RNase 7 antibody [4C4] (ab205565)
      All lanes : Anti-RNase 7 antibody [4C4] (ab205565) at 2 µg/ml

      Lane 1 : CHOEBNALT85 cell line lysate; non-reduced conditions at 10 µl
      Lane 2 : CHOEBNALT85 cell line lysate; reduced conditions at 10 µl
      Lane 3 : Protein ladder

      Predicted band size: 17 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-RNase 7 antibody [4C4] (ab205565)
      Immunocytochemistry/ Immunofluorescence - Anti-RNase 7 antibody [4C4] (ab205565)

      Immunofluorescence analysis of  U2OS cells labeling RNAase 7 with ab205565 at 0.5 µg/mL. Nuclei were counterstained by DAPI. Left image; U2OS cells expressing RNAase 7. Right image; Negative control (non-transfected U2OS cells).

    References

    This product has been referenced in:

    • Gazi U  et al. Tonsillar antimicrobial peptide (AMP) expression profiles of periodic fever, aphthous stomatitis, pharyngitis, cervical adenitis (PFAPA) patients. Int J Pediatr Otorhinolaryngol 110:100-104 (2018). IHC-P ; Human . Read more (PubMed: 29859567) »
    See 1 Publication for this product

    Publishing research using ab205565? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RNase 7 antibody [4C4]

     Good
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (skin)
    Permeabilization
    No
    Specification
    skin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 30 2019

    Western blot abreview for Anti-RNase 7 antibody [4C4]

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Monocytes)
    Gel Running Conditions
    Non-reduced Denaturing (4-20%)
    Loading amount
    15 µg
    Specification
    Monocytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 30 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RNase 7 antibody [4C4]

     Good
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrat buffer pH6,0
    Permeabilization
    No
    Specification
    skin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 30 2019

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