Key features and details
- Mouse monoclonal [4C4] to RNase 7
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-RNase 7 antibody [4C4]
See all RNase 7 primary antibodies
DescriptionMouse monoclonal [4C4] to RNase 7
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human RNase 7. Recombinant Human RNase 7 protein produced by CHO-based proprietary suspension cell line.
Database link: Q9H1E1
- CHOEBNALT85 cell line lysate. U2OS cells expressing RNase 7. Human skin tissue.
Protein previously labeled as Ribonuclease 7.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab205565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 17 kDa.
WB in non-reduced conditions.
|ICC/IF||Use a concentration of 0.5 - 20 µg/ml.|
FunctionExhibits a potent RNase activity. Has broad-spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium.
Tissue specificityExpressed in various epithelial tissues including skin, respiratory tract, genito-urinary tract and, at a low level, in the gut. Expressed in liver, kidney, skeletal muscle and heart.
Sequence similaritiesBelongs to the pancreatic ribonuclease family.
- Information by UniProt
- EC 3.1.27.- antibody
- MGC133220 antibody
- Ribonuclease 7 antibody
Immunohistochemical analysis of paraffin-embedded human skin tissue tissue labeling RNAase 7 with ab205565 at 1/500 dilution. Biotinylated anti-mouse antibody was used as secondary antibody. Streptavidin-HRP conjugate was used for visualization.
All lanes : Anti-RNase 7 antibody [4C4] (ab205565) at 2 µg/ml
Lane 1 : CHOEBNALT85 cell line lysate; non-reduced conditions at 10 µl
Lane 2 : CHOEBNALT85 cell line lysate; reduced conditions at 10 µl
Lane 3 : Protein ladder
Predicted band size: 17 kDa
Immunofluorescence analysis of U2OS cells labeling RNAase 7 with ab205565 at 0.5 µg/mL. Nuclei were counterstained by DAPI. Left image; U2OS cells expressing RNAase 7. Right image; Negative control (non-transfected U2OS cells).
ab205565 has been referenced in 1 publication.
- Gazi U et al. Tonsillar antimicrobial peptide (AMP) expression profiles of periodic fever, aphthous stomatitis, pharyngitis, cervical adenitis (PFAPA) patients. Int J Pediatr Otorhinolaryngol 110:100-104 (2018). IHC-P ; Human . PubMed: 29859567