Anti-RNase 7 antibody [4C4] (ab205565)
Key features and details
- Mouse monoclonal [4C4] to RNase 7
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-RNase 7 antibody [4C4]
See all RNase 7 primary antibodies -
Description
Mouse monoclonal [4C4] to RNase 7 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human RNase 7. Recombinant Human RNase 7 protein produced by CHO-based proprietary suspension cell line.
Database link: Q9H1E1 -
Positive control
- CHOEBNALT85 cell line lysate. U2OS cells expressing RNase 7. Human skin tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4C4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use a concentration of 5 - 20 µg/ml.
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WB | (1) |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 17 kDa.
WB in non-reduced conditions. |
ICC/IF | (1) |
Use a concentration of 0.5 - 20 µg/ml.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 17 kDa. WB in non-reduced conditions. |
ICC/IF
Use a concentration of 0.5 - 20 µg/ml. |
Target
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Function
Exhibits a potent RNase activity. Has broad-spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium. -
Tissue specificity
Expressed in various epithelial tissues including skin, respiratory tract, genito-urinary tract and, at a low level, in the gut. Expressed in liver, kidney, skeletal muscle and heart. -
Sequence similarities
Belongs to the pancreatic ribonuclease family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 84659 Human
- Omim: 612484 Human
- SwissProt: Q9H1E1 Human
- Unigene: 525206 Human
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Alternative names
- EC 3.1.27.- antibody
- MGC133220 antibody
- Ribonuclease 7 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human skin tissue tissue labeling RNAase 7 with ab205565 at 1/500 dilution. Biotinylated anti-mouse antibody was used as secondary antibody. Streptavidin-HRP conjugate was used for visualization.
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All lanes : Anti-RNase 7 antibody [4C4] (ab205565) at 2 µg/ml
Lane 1 : CHOEBNALT85 cell line lysate; non-reduced conditions at 10 µl
Lane 2 : CHOEBNALT85 cell line lysate; reduced conditions at 10 µl
Lane 3 : Protein ladder
Predicted band size: 17 kDa -
Immunofluorescence analysis of U2OS cells labeling RNAase 7 with ab205565 at 0.5 µg/mL. Nuclei were counterstained by DAPI. Left image; U2OS cells expressing RNAase 7. Right image; Negative control (non-transfected U2OS cells).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab205565 has been referenced in 1 publication.
- Gazi U et al. Tonsillar antimicrobial peptide (AMP) expression profiles of periodic fever, aphthous stomatitis, pharyngitis, cervical adenitis (PFAPA) patients. Int J Pediatr Otorhinolaryngol 110:100-104 (2018). IHC-P ; Human . PubMed: 29859567