Anti-RNase 7 antibody (ab81470)

Submit a review Q&A (1)

Key features and details

  • Rabbit polyclonal to RNase 7
  • Suitable for: IHC-Fr, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-RNase 7 antibody
    See all RNase 7 primary antibodies

  • Description

    Rabbit polyclonal to RNase 7

  • Host species

    Rabbit

  • Specificity

    ab81470 reacts with a 17 kDa RNase 7 protein. It also cross reacts with Ribonuclease 8 protein.

  • Tested applications

    Suitable for: IHC-Fr, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human RNase 7 (N terminal).

  • General notes

    Protein previously labeled as Ribonuclease 7.

Properties

Applications

Our Abpromise guarantee covers the use of ab81470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200 - 1/1000.
WB 1/500 - 1/5000. Predicted molecular weight: 17 kDa.

Target

  • Function

    Exhibits a potent RNase activity. Has broad-spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium.

  • Tissue specificity

    Expressed in various epithelial tissues including skin, respiratory tract, genito-urinary tract and, at a low level, in the gut. Expressed in liver, kidney, skeletal muscle and heart.

  • Sequence similarities

    Belongs to the pancreatic ribonuclease family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • EC 3.1.27.- antibody
      • MGC133220 antibody
      • Ribonuclease 7 antibody
      • ribonuclease, RNase A family, 7 antibody
      • RNAS7_HUMAN antibody
      • RNase 7 antibody
      • RNASE7 antibody
      • SAP 2 antibody
      • SAP-2 antibody
      • Skin derived antimicrobial protein 2 antibody
      • Skin-derived antimicrobial protein 2 antibody
      see all

    Images

    • Western blot - Anti-RNase 7 antibody (ab81470)
      Western blot - Anti-RNase 7 antibody (ab81470)
      All lanes : Anti-RNase 7 antibody (ab81470) at 1/500 dilution

      Lane 1 : Human small intestine tissue lysate - total protein (ab29276)
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Predicted band size: 17 kDa
      Observed band size: 17 kDa
      Additional bands at: 37 kDa, 40 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes

    References (0)

    Publishing research using ab81470? Please let us know so that we can cite the reference in this datasheet.

    ab81470 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Submit a review Submit a question

    Question

    I'm interested in your anti-RNase 7 antibody (ab81470) as an ELISA
    antibody for research.
    Did you test it for ELISA? And if you did: How are the results, and if
    it hadn't been tested: Would you consider it sensible to use it for
    ELISA?

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    Answer

    Thank you very much for your interest in ab81470.

    To our knowledge, ab81470 has not been tested in ELISA. Therefore, I can offer a discount off a future purchase if you buy ab81470 now, test it in ELISA and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab81470 in ELISA. I will then send a discount code. This code must be issued before purchasing ab81470 so please wait for my reply before ordering.

    2. Purchase ab81470 either by phone, fax, or online (www.abcam.com).

    3. Test it in ELISA.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab81470 turns out to be unsuitable for ELISA, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com