Anti-RNase 7 antibody (ab81470)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RNase 7 antibody
See all RNase 7 primary antibodies -
Description
Rabbit polyclonal to RNase 7 -
Host species
Rabbit -
Specificity
ab81470 reacts with a 17 kDa RNase 7 protein. It also cross reacts with Ribonuclease 8 protein.
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Tested applications
Suitable for: IHC-Fr, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human RNase 7 (N terminal).
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General notes
Protein previously labeled as Ribonuclease 7.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab81470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr | 1/200 - 1/1000. | |
WB | 1/500 - 1/5000. Predicted molecular weight: 17 kDa. |
Target
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Function
Exhibits a potent RNase activity. Has broad-spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium. -
Tissue specificity
Expressed in various epithelial tissues including skin, respiratory tract, genito-urinary tract and, at a low level, in the gut. Expressed in liver, kidney, skeletal muscle and heart. -
Sequence similarities
Belongs to the pancreatic ribonuclease family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 84659 Human
- Omim: 612484 Human
- SwissProt: Q9H1E1 Human
- Unigene: 525206 Human
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Alternative names
- EC 3.1.27.- antibody
- MGC133220 antibody
- Ribonuclease 7 antibody
see all
Images
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All lanes : Anti-RNase 7 antibody (ab81470) at 1/500 dilution
Lane 1 : Human small intestine tissue lysate - total protein (ab29276)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 17 kDa
Observed band size: 17 kDa
Additional bands at: 37 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Datasheets and documents
References
ab81470 has not yet been referenced specifically in any publications.