Overview

  • Product name
  • Description
    Rabbit polyclonal to RNASEH2B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RNASEH2B aa 136-166 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q5TBB1

  • Positive control
    • CEM cell lysate, Human lymph tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab175066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 35 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.
  • Tissue specificity
    Widely expressed.
  • Involvement in disease
    Defects in RNASEH2B are a cause of Aicardi-Goutieres syndrome type 2 (AGS2) [MIM:610181]. A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood.
  • Sequence similarities
    Belongs to the RNase H2 subunit B family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGS2 antibody
    • Aicardi-Goutieres syndrome 2 protein antibody
    • Deleted in lymphocytic leukemia 8 antibody
    • DLEU8 antibody
    • FLJ11712 antibody
    • Ribonuclease H2 subunit B antibody
    • Ribonuclease HI subunit B antibody
    • RNase H2 subunit B antibody
    • Rnaseh2b antibody
    • RNH2B_HUMAN antibody
    see all

Images

  • Anti-RNASEH2B antibody (ab175066) at 1/100 dilution + CEM cell lysate at 35 µg

    Predicted band size: 35 kDa

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human lymph tissue, labeling RNASEH2B using ab175066 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab175066 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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